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Heterologous expression and characterization of a xylanase and xylosidase from white rot fungi and their application in synergistic hydrolysis of lignocellulose
- Source :
- Chemosphere. 212:24-33
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Endo-xylanase and β-xylosidase are the major enzymes for hemicellulose hydrolysis, which play a significant role in biomass conversion. In our previous work, the white-rot fungi Pleurotus ostreatus HAUCC 162 and Irpex lacteus CD2 were demonstrated to have strong ability in lignocellulose degradation, and the related lignin degradation enzymes were characterized. However, little was known about their hemicellulases. In this work, a novel endo-1, 4-xylanase and a β-xylosidase from Pleurotus ostreatus HAUCC 162 and Irpex lacteus CD2 were heterologously expressed and characterized. The optima of pH and temperature were 5.0 and 55 °C for rXyn162, and 6.5 and 30 °C for rXylCD2. rXyn162 showed high tolerance to metal ions such as Ca2+, Cr3+, Zn2+, Na+, and Al3+. The recombinant rXyn162 and rXylCD2 exhibited synergistic hydrolysis of oat spelts xylan and sodium hydroxide pretreated cornstalk (SHPC), where the degree of synergy (DS) was 2.26 for SHPC hydrolysis. MALDI-TOF-MS and HPLC analysis showed that xylooligosaccharides (XOS) with small degrees of polymerization (DP2-DP4) were the major XOS hydrolyzate during SHPC degradation by rXyn162 and rXylCD2. In addition, rXyn162 and rXylCD2 could efficiently improve the hydrolysis of SHPC by commercial cellulase. The present study suggested the potential application of rXyn162 and rXylCD2 in the field of biomass pretreatment and biofuel production.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Environmental Engineering
Glycoside Hydrolases
Health, Toxicology and Mutagenesis
Irpex lacteus
Oligosaccharides
Glucuronates
Cellulase
Pleurotus
Lignin
01 natural sciences
Pichia
03 medical and health sciences
chemistry.chemical_compound
Hydrolysis
010608 biotechnology
Environmental Chemistry
Hemicellulose
Biomass
Food science
Endo-1,4-beta Xylanases
biology
Public Health, Environmental and Occupational Health
General Medicine
General Chemistry
biology.organism_classification
Pollution
Xylan
Xylosidases
030104 developmental biology
chemistry
Sodium hydroxide
Xylanase
biology.protein
Pleurotus ostreatus
Subjects
Details
- ISSN :
- 00456535
- Volume :
- 212
- Database :
- OpenAIRE
- Journal :
- Chemosphere
- Accession number :
- edsair.doi.dedup.....83dde709ec6e8707e7d3648dc3ae2307