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Lipopolysaccharide from Gut-Associated Lymphoid-Tissue-Resident Alcaligenes faecalis: Complete Structure Determination and Chemical Synthesis of Its Lipid A

Authors :
Angelo Palmigiano
Naoko Shibata
Flaviana Di Lorenzo
Tomoya Uto
Luisa Sturiale
Atsushi Shimoyama
Immacolata Speciale
Seiji Masui
Keisuke Mizote
Koji Hosomi
Haruki Yamaura
Yukari Fujimoto
Jun Kunisawa
Domenico Garozzo
Alba Silipo
Koichi Fukase
Hiroshi Kiyono
Kazuya Kabayama
Molly D. Pither
Antonio Molinaro
Shimoyama, A.
Di Lorenzo, F.
Yamaura, H.
Mizote, K.
Palmigiano, A.
Pither, M. D.
Speciale, I.
Uto, T.
Masui, S.
Sturiale, L.
Garozzo, D.
Hosomi, K.
Shibata, N.
Kabayama, K.
Fujimoto, Y.
Silipo, A.
Kunisawa, J.
Kiyono, H.
Molinaro, A.
Fukase, K.
Source :
Angewandte Chemie (Int. ed., Print) 60 (2021): 10023–10031. doi:10.1002/anie.202012374, info:cnr-pdr/source/autori:Shimoyama, Atsushi; Di Lorenzo, Flaviana; Yamaura, Haruki; Mizote, Keisuke; Palmigiano, Angelo; Pither, Molly D.; Speciale, Immacolata; Uto, Tomoya; Masui, Seiji; Sturiale, Luisa; Garozzo, Domenico; Hosomi, Koji; Shibata, Naoko; Kabayama, Kazuya; Fujimoto, Yukari; Silipo, Alba; Kunisawa, Jun; Kiyono, Hiroshi; Molinaro, Antonio; Fukase, Koichi/titolo:Lipopolysaccharide from Gut-Associated Lymphoid-Tissue-Resident Alcaligenes faecalis: Complete Structure Determination and Chemical Synthesis of Its Lipid A/doi:10.1002%2Fanie.202012374/rivista:Angewandte Chemie (Int. ed., Print)/anno:2021/pagina_da:10023/pagina_a:10031/intervallo_pagine:10023–10031/volume:60, Angewandte Chemie International Edition, Angewandte Chemie (International Ed. in English)
Publication Year :
2021
Publisher :
Wiley-VCH., Weinheim, Germania, 2021.

Abstract

Alcaligenes faecalis is the predominant Gram‐negative bacterium inhabiting gut‐associated lymphoid tissues, Peyer's patches. We previously reported that an A. faecalis lipopolysaccharide (LPS) acted as a weak agonist for Toll‐like receptor 4 (TLR4)/myeloid differentiation factor‐2 (MD‐2) receptor as well as a potent inducer of IgA without excessive inflammation, thus suggesting that A. faecalis LPS might be used as a safe adjuvant. In this study, we characterized the structure of both the lipooligosaccharide (LOS) and LPS from A. faecalis. We synthesized three lipid A molecules with different degrees of acylation by an efficient route involving the simultaneous introduction of 1‐ and 4′‐phosphates. Hexaacylated A. faecalis lipid A showed moderate agonistic activity towards TLR4‐mediated signaling and the ability to elicit a discrete interleukin‐6 release in human cell lines and mice. It was thus found to be the active principle of the LOS/LPS and a promising vaccine adjuvant candidate.<br />A host–microbe chemical ecology study revealed an effective and safe immunomodulator from Alcaligenes faecalis resident in gut‐associated lymphoid tissues, Peyer's patches (see picture). The complete structures of both the lipooligosaccharide and the lipopolysaccharide from A. faecalis were characterized. Furthermore, A. faecalis lipid A molecules were synthesized with varying degrees of acylation and found to be a promising vaccine adjuvant candidate.

Details

Language :
English
Database :
OpenAIRE
Journal :
Angewandte Chemie (Int. ed., Print) 60 (2021): 10023–10031. doi:10.1002/anie.202012374, info:cnr-pdr/source/autori:Shimoyama, Atsushi; Di Lorenzo, Flaviana; Yamaura, Haruki; Mizote, Keisuke; Palmigiano, Angelo; Pither, Molly D.; Speciale, Immacolata; Uto, Tomoya; Masui, Seiji; Sturiale, Luisa; Garozzo, Domenico; Hosomi, Koji; Shibata, Naoko; Kabayama, Kazuya; Fujimoto, Yukari; Silipo, Alba; Kunisawa, Jun; Kiyono, Hiroshi; Molinaro, Antonio; Fukase, Koichi/titolo:Lipopolysaccharide from Gut-Associated Lymphoid-Tissue-Resident Alcaligenes faecalis: Complete Structure Determination and Chemical Synthesis of Its Lipid A/doi:10.1002%2Fanie.202012374/rivista:Angewandte Chemie (Int. ed., Print)/anno:2021/pagina_da:10023/pagina_a:10031/intervallo_pagine:10023–10031/volume:60, Angewandte Chemie International Edition, Angewandte Chemie (International Ed. in English)
Accession number :
edsair.doi.dedup.....83cc032fb2183f8dfbb9f37acafa1ed9
Full Text :
https://doi.org/10.1002/anie.202012374