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LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway
- Source :
- Experimental cell research. 396(1)
- Publication Year :
- 2020
-
Abstract
- The proliferation and differentiation of myoblast cells are regulated by the fibroblast growth factor receptor (FGFR) signaling pathway. Although the regulation of FGFR signaling cascades has been widely investigated, the inhibitory mechanism that particularly function in skeletal muscle myogenesis remains obscure. In this study, we determined that LRTM1, an inhibitory regulator of the FGFR signaling pathway, negatively modulates the activation of ERK and promotes the differentiation of myoblast cells. LRTM1 is dynamically expressed during myoblast differentiation and skeletal muscle regeneration after injury. In mouse myoblast C2C12 cells, knockout (KO) of Lrtm1 significantly prevents the differentiation of myoblast cells; this effect is associated with the reduction of MyoD transcriptional activity and the overactivation of ERK kinase. Notably, further studies demonstrated that LRTM1 associates with p52Shc and inhibits the recruitment of p52Shc to FGFR1. Taken together, our findings identify a novel negative regulator of FGFR1, which plays an important role in regulating the differentiation of myoblast cells.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
Src Homology 2 Domain-Containing, Transforming Protein 1
Nerve Tissue Proteins
Biology
MyoD
Muscle Development
Cardiotoxins
p38 Mitogen-Activated Protein Kinases
Cell Line
Myoblasts
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Myocyte
Animals
Humans
Receptor, Fibroblast Growth Factor, Type 1
Muscle, Skeletal
Cell Proliferation
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Myogenesis
Fibroblast growth factor receptor 1
Gene Expression Profiling
Skeletal muscle
Membrane Proteins
Cell Differentiation
Cell Biology
musculoskeletal system
Cell biology
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
Fibroblast growth factor receptor
030220 oncology & carcinogenesis
Myogenin
Signal transduction
tissues
Signal Transduction
Subjects
Details
- ISSN :
- 10902422
- Volume :
- 396
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Experimental cell research
- Accession number :
- edsair.doi.dedup.....83abd99f63d0cc030387e67a64a936bf