Back to Search
Start Over
Overexpression of glucose-6-phosphate dehydrogenase extends the life span of Drosophila melanogaster
- Source :
- The Journal of biological chemistry. 283(47)
- Publication Year :
- 2008
-
Abstract
- The redox state of tissues tends to become progressively more prooxidizing during the aging process. The hypothesis tested in this study was that enhancement of reductive capacity by overexpression of glucose-6-phosphate dehydrogenase (G6PD), a key enzyme for NADPH biosynthesis, could protect against oxidative stress and extend the life span of transgenic Drosophila melanogaster. Overexpression of G6PD was achieved by combining a UAS-G6PD responder transgene at one of four independent loci with either a broad expression (armadillo-GAL4, Tubulin-GAL4, C23-GAL4, and da-GAL4) or a neuronal driver (D42-GAL4 and Appl-GAL4). The mean life spans of G6PD overexpressor flies were extended, in comparison with driver and responder controls, as follows: armadillo-GAL4 (up to 38%), Tubulin-GAL4 (up to 29%), C23-GAL4 (up to 27%), da-GAL4 (up to 24%), D42-GAL4 (up to 18%), and Appl-GAL4 (up to 16%). The G6PD enzymatic activity was increased, as were the levels of NADPH, NADH, and the GSH/GSSG ratio. Resistance to experimental oxidative stress was enhanced. Furthermore, metabolic rates and fertility were essentially the same in G6PD overexpressors and control flies. Collectively, the results demonstrate that enhancement of the NADPH biosynthetic capability can extend the life span of a relatively long-lived strain of flies, which supports the oxidative stress hypothesis of aging.
- Subjects :
- Male
Aging
animal structures
media_common.quotation_subject
Transgene
Longevity
Dehydrogenase
Glucosephosphate Dehydrogenase
medicine.disease_cause
Biochemistry
Models, Biological
chemistry.chemical_compound
Oxygen Consumption
medicine
Glucose-6-phosphate dehydrogenase
Animals
Transgenes
Molecular Biology
media_common
chemistry.chemical_classification
biology
fungi
Cell Biology
Glutathione
biology.organism_classification
Cell biology
Luminescent Proteins
Oxidative Stress
Metabolism and Bioenergetics
Enzyme
Drosophila melanogaster
chemistry
Microscopy, Fluorescence
Models, Chemical
Oxidation-Reduction
Oxidative stress
NADP
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 283
- Issue :
- 47
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....837afbd39875127dfcb4f86402ff6894