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Chromosomal localization and characterization of c-abl in the t(6;9) of acute nonlymphocytic leukemia
- Source :
- Proceedings of the National Academy of Sciences. 82:8742-8746
- Publication Year :
- 1985
- Publisher :
- Proceedings of the National Academy of Sciences, 1985.
-
Abstract
- Acute nonlymphocytic leukemia associated with the chromosomal translocation t(6;9)(p23;q34) is an entity that is frequently associated with basophilia, which it shares with chronic myelogenous leukemia. The breakpoint on chromosome 9, q34, appears to be cytogenetically identical in both malignancies and is the site of the cellular oncogene c-abl. We investigated the role of c-abl in cells from two patients with the t(6;9) using in situ chromosomal hybridization, Southern hybridization, and in vitro phosphorylation. We showed that c-abl is not translocated from chromosome 9, resulting in a breakpoint that is on the 3' side of this gene. The t(6;9) translocation does not appear to result in the production of an aberrantly sized protein product or in the acquisition of in vitro tyrosine kinase activity. This is in direct contrast to the findings in chronic myelogenous leukemia, in which c-abl is translocated, leading to the production of a structurally altered c-abl protein with activated tyrosine kinase. Lastly, we demonstrated that the cells of one patient contain sequences from chromosome 9 inserted at the junction of a reciprocal translocation between chromosomes 4 and 10 on the 4q+ chromosome. This insertion, which is at least 100 kilobase pairs in length, represents a duplication and translocation of the protein coding region of c-abl.
- Subjects :
- Adult
Male
Chromosomal translocation
Chromosome 9
Biology
Translocation, Genetic
hemic and lymphatic diseases
Proto-Oncogenes
medicine
Humans
Chromosomes, Human, 4-5
Chromosomes, Human, 6-12 and X
Leukemia
Multidisciplinary
ABL
Breakpoint
Chromosome Mapping
Nucleic Acid Hybridization
Gene rearrangement
Protein-Tyrosine Kinases
medicine.disease
Molecular biology
Basophilia
Child, Preschool
Research Article
Chronic myelogenous leukemia
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....83774cd77d0ffb386ddc5f2a8c8fc896