Back to Search
Start Over
A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol
- Source :
- Journal of visualized experiments : JoVE. (131)
- Publication Year :
- 2018
-
Abstract
- Electronic cigarettes are the most popular tobacco product among middle and high schoolers and are the most popular alternative tobacco product among adults. High quality, reproducible research on the consequences of electronic cigarette use is essential for understanding emerging public health concerns and crafting evidence based regulatory policy. While a growing number of papers discuss electronic cigarettes, there is little consistency in methods across groups and very little consensus on results. Here, we describe a programmable laboratory device that can be used to create extracts of conventional cigarette smoke and electronic cigarette aerosol. This protocol details instructions for the assembly and operation of said device, and demonstrates the use of the generated extract in two sample applications: an in vitro cell viability assay and gas-chromatography mass-spectrometry. This method provides a tool for making direct comparisons between conventional cigarettes and electronic cigarettes, and is an accessible entry point into electronic cigarette research.
- Subjects :
- 0301 basic medicine
Computer science
General Chemical Engineering
Internet privacy
Bioengineering
Electronic Nicotine Delivery Systems
Laboratory device
General Biochemistry, Genetics and Molecular Biology
law.invention
03 medical and health sciences
0302 clinical medicine
law
Cigarette smoke
Humans
030212 general & internal medicine
Two sample
Aerosols
General Immunology and Microbiology
business.industry
General Neuroscience
Smoking
Regulatory policy
Electronic Cigarette Use
Microcontroller
030104 developmental biology
business
Electronic cigarette
Tobacco product
Subjects
Details
- ISSN :
- 1940087X
- Issue :
- 131
- Database :
- OpenAIRE
- Journal :
- Journal of visualized experiments : JoVE
- Accession number :
- edsair.doi.dedup.....836cf3380f534ca097033df21582b0d1