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The subiculum is a patchwork of discrete subregions
- Source :
- eLife, eLife, Vol 7 (2018)
- Publication Year :
- 2018
- Publisher :
- eLife Sciences Publications, Ltd, 2018.
-
Abstract
- In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here, we investigated the extent and organizational principles governing pyramidal cell heterogeneity throughout the mouse subiculum. Using single-cell RNA-seq, we find that the subiculum pyramidal cell population can be deconstructed into eight separable subclasses. These subclasses were mapped onto abutting spatial domains, ultimately producing a complex laminar and columnar organization with heterogeneity across classical dorsal-ventral, proximal-distal, and superficial-deep axes. We further show that these transcriptomically defined subclasses correspond to differential protein products and can be associated with specific projection targets. This work deconstructs the complex landscape of subiculum pyramidal cells into spatially segregated subclasses that may be observed, controlled, and interpreted in future experiments.
- Subjects :
- Male
0301 basic medicine
Cell type
Mouse
QH301-705.5
hippocampus
Science
Population
Hippocampus
Mice, Transgenic
Hippocampal formation
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Biology (General)
education
CA1 Region, Hippocampal
pyramidal cell
education.field_of_study
General Immunology and Microbiology
Sequence Analysis, RNA
Gene Expression Profiling
Pyramidal Cells
General Neuroscience
S100 Proteins
Subiculum
Reproducibility of Results
food and beverages
cell type
General Medicine
Tools and Resources
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
nervous system
subiculum
Medicine
RNA-seq
Single-Cell Analysis
Pyramidal cell
Transcriptome
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 2050084X
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....83695a5aad378e356a194ea828101d26