Back to Search
Start Over
Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana
- Source :
- Journal of Veterinary Diagnostic Investigation, Vol. 14, N° 3 : 240-242, 2002, Repositorio UNA, Universidad Nacional de Costa Rica, instacron:UNA
- Publication Year :
- 2002
- Publisher :
- SAGE Publications, 2002.
-
Abstract
- A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms in the Poa´s region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95% confidence interval [CI]: 0.8587–0.9155) and 0.6912 (95% CI: 0.6246–0.7577) for the VSV-NJ and VSV-IN tests, respectively. These results indicate good to excellent agreement between the 2 tests under these conditions. Ensayo inmunoabsorbente ligado a enzimas competitivo (C-ELISA) para la detección de anticuerpos contra El virus de la estomatitis vesicular de Nueva Jersey (VSV-NJ) y el virus de la estomatitis vesicular de Indiana (VSV-IN) se compararon con la prueba de seroneutralización (SNT) utilizando 1106 muestras de suero obtenidas de ganado lechero en granjas de estudio centinela en la región del Poa de Costa Rica. Los coeficientes Kappa entre el C-ELISA y el SNT fueron 0,8871 (95% intervalo de confianza [IC]: 0,8587–0,9155) y 0,6912 (IC 95 %: 0,6246–0,7577) para las pruebas VSV-NJ y VSV-IN, respectivamente. Estos resultados indican una concordancia de buena a excelente entre las 2 pruebas en estas condiciones. Universidad Nacional, Costa Rica Escuela de Medicina Veterinaria
- Subjects :
- 0301 basic medicine
Serotype
040301 veterinary sciences
VIRUSES
viruses
VESICULAR STOMATITIS VIRUS
030106 microbiology
Enzyme-Linked Immunosorbent Assay
Antibodies, Viral
HORSES
Vesicular stomatitis Indiana virus
MEDICINA VETERINARIA
Virus
Animal Diseases
0403 veterinary science
03 medical and health sciences
Vesicular Stomatitis
Neutralization Tests
Rhabdoviridae Infections
Animals
Medicine
COSTA RICA
Serotyping
Mononegavirales
Stomatitis
General Veterinary
biology
business.industry
CABALLOS
Vesiculovirus
04 agricultural and veterinary sciences
ESTOMATITIS
Rhabdoviridae
biology.organism_classification
Virology
stomatognathic diseases
EQUINE
Vesicular stomatitis virus
Animals, Domestic
biology.protein
ELISA
Antibody
business
Subjects
Details
- ISSN :
- 19434936 and 10406387
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Diagnostic Investigation
- Accession number :
- edsair.doi.dedup.....83401277f9f2549a04fe7108036a9f2a
- Full Text :
- https://doi.org/10.1177/104063870201400309