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Expression of a human gene for interferon in yeast
- Source :
- Nature. 293(5835)
- Publication Year :
- 1981
-
Abstract
- A DNA sequence coding for mature human leukocyte interferon D (LeIF-D) was linked with DNA fragments of the 5'-flanking sequences of the Saccharomyces cerevisiae (yeast) alcohol dehydrogenase I gene in a plasmid capable of autonomous replication and selection in both yeast and Escherichia coli. Yeast cells transformed by these plasmids synthesize up to 1 x 10(6) molecules of biologically active LeIF-D per cell.
- Subjects :
- Transcription, Genetic
Autonomously replicating sequence
Genetic Linkage
Saccharomyces cerevisiae
DNA, Recombinant
Biology
law.invention
chemistry.chemical_compound
Plasmid
Transformation, Genetic
law
Operon
Humans
Genomic library
Cloning, Molecular
Gene
Multidisciplinary
biology.organism_classification
Molecular biology
Yeast
Alcohol Oxidoreductases
chemistry
Gene Expression Regulation
Genes
Protein Biosynthesis
Recombinant DNA
Interferons
DNA
Plasmids
Subjects
Details
- ISSN :
- 00280836
- Volume :
- 293
- Issue :
- 5835
- Database :
- OpenAIRE
- Journal :
- Nature
- Accession number :
- edsair.doi.dedup.....8339584c0ab86c4422a4ce17910e1772