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Extruded Bioreactor Perfusion Culture Supports the Chondrogenic Differentiation of Human Mesenchymal Stem/Stromal Cells in 3D Porous Poly(ɛ‐Caprolactone) Scaffolds
- Source :
- Biotechnology Journal. 15:1900078
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Novel bioengineering strategies for the ex vivo fabrication of native-like tissue-engineered cartilage are crucial for the translation of these approaches to clinically manage highly prevalent and debilitating joint diseases. Bioreactors that provide different biophysical stimuli have been used in tissue engineering approaches aimed at enhancing the quality of the cartilage tissue generated. However, such systems are often highly complex, expensive, and not very versatile. In the current study, a novel, cost-effective, and customizable perfusion bioreactor totally fabricated by additive manufacturing (AM) is proposed for the study of the effect of fluid flow on the chondrogenic differentiation of human bone-marrow mesenchymal stem/stromal cells (hBMSCs) in 3D porous poly(ɛ-caprolactone) (PCL) scaffolds. hBMSCs are first seeded and grown on PCL scaffolds and hBMSC-PCL constructs are then transferred to 3D-extruded bioreactors for continuous perfusion culture under chondrogenic inductive conditions. Perfused constructs show similar cell metabolic activity and significantly higher sulfated glycosaminoglycan production (≈1.8-fold) in comparison to their non-perfused counterparts. Importantly, perfusion bioreactor culture significantly promoted the expression of chondrogenic marker genes while downregulating hypertrophy. This work highlights the potential of customizable AM platforms for the development of novel personalized repair strategies and more reliable in vitro models with a wide range of applications.
- Subjects :
- 0106 biological sciences
Stromal cell
Biocompatible Materials
01 natural sciences
Applied Microbiology and Biotechnology
Lactones
Bioreactors
Perfusion Culture
Tissue engineering
010608 biotechnology
medicine
Bioreactor
Humans
Caproates
Cells, Cultured
Glycosaminoglycans
Tissue Engineering
Tissue Scaffolds
Chemistry
Cartilage
010401 analytical chemistry
Mesenchymal stem cell
technology, industry, and agriculture
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
equipment and supplies
Chondrogenesis
0104 chemical sciences
Cell biology
Perfusion
medicine.anatomical_structure
Molecular Medicine
Porosity
Ex vivo
Subjects
Details
- ISSN :
- 18607314 and 18606768
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Biotechnology Journal
- Accession number :
- edsair.doi.dedup.....83392fd2fd56391b5ee933561c9f506f