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Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia
- Publication Year :
- 2016
- Publisher :
- American Society for Microbiology, 2016.
-
Abstract
- Contagious bovine pleuropneumonia (CBPP) is a severe respiratory disease that is widespread in sub-Saharan Africa. It is caused by Mycoplasma mycoides subsp. mycoides , a bacterium belonging to the Mycoplasma mycoides cluster. In the absence of an efficient CBPP vaccine, improved and easy-to-use diagnostic assays for recurrent testing combined with isolation and treatment of positive animals represent an option for CBPP control in Africa. Here we describe the comprehensive screening of 17 immunogenic Mycoplasma mycoides subsp. mycoides proteins using well-characterized bovine sera for the development of a novel cocktail enzyme-linked immunosorbent assay (ELISA) for laboratory use. Two recombinant Mycoplasma immunogens, MSC_0136 and MSC_0636, were used to set up a standardized cocktail ELISA protocol. According to the results from more than 100 serum samples tested, the sensitivity and specificity of the novel cocktail ELISA were 85.6% and 96.4%, respectively, with an overall diagnostic accuracy comparable to that of the Office International des Epizooties (OIE)-prescribed serological assays. In addition, we provide a proof of principle for a field-applicable, easy-to-use commercially produced prototype lateral-flow test for rapid (
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Time Factors
030106 microbiology
Cattle Diseases
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Sensitivity and Specificity
Chromatography, Affinity
law.invention
Serology
Microbiology
Clinical Veterinary Microbiology
03 medical and health sciences
Contagious bovine pleuropneumonia
law
medicine
Animals
Pleuropneumonia, Contagious
Africa South of the Sahara
chemistry.chemical_classification
biology
630 Agriculture
Mycoplasma mycoides
Mycoplasma
biology.organism_classification
medicine.disease
Virology
Antibodies, Bacterial
Enzyme
chemistry
Recombinant DNA
Pleuropneumonia
biology.protein
Cattle
Antibody
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....83307b71dbd7656c374fc546e5be8714
- Full Text :
- https://doi.org/10.7892/boris.122104