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Enhanced Detection of Human Plasma Proteins on Nanostructured Silver Surfaces
- Source :
- Nanomaterials and Nanotechnology, Nanomaterials and Nanotechnology, Vol 3, Iss, p 17 (2013), Volume 3, Issue Godište 2013, Scopus-Elsevier
- Publication Year :
- 2013
- Publisher :
- InTech, 2013.
-
Abstract
- In chemical and medical research, recent methods combine the tools of nanotechnology, chemistry and biology in a way that introduces the most modern processes to current medical practice. The main blood plasma proteins – albumin and globulin and their amino acid sequences, are carriers of important information about human health. In this paper we employed silver nanostructured surfaces prepared by electrodeposition. Consequently, electrochemical deposition is introduced as a convenient, fast and cost-effective method for the preparation of metallic nanostructures with required morphology. Silver nanostructured surfaces were applied as the templates for Surface Enhanced Raman Spectroscopy (SERS) of albumin and globulin in the role of model analytes. We also studied the effect of a working electrode polishing process on electrodeposition and identification of proteins. The aqueous solutions of albumin and globulin were applied onto these Ag nanostructured substrates separately. An analytical signal enhancement factor of 3.6×102 was achieved for a band with a Raman shift of 2104cm-1 for globulin deposited onto silver nanostructured film on unpolished stainless steel substrate. The detection limit was 400μg/mL. Plasma or serum could present a preferable material for noninvasive cancer disease diagnosis using the SERS method.
- Subjects :
- Materials science
Electrochemical Deposition
SERS
Albumin
Inorganic chemistry
Medical practice
Nanotechnology
electrochemical deposition
nanostructured surfaces
albumin
globulin, surface enhanced Raman spectroscopy (SERS)
Medical research
Electronic, Optical and Magnetic Materials
Human plasma
Globulin
lcsh:Technology (General)
Ceramics and Composites
lcsh:T1-995
Electrical and Electronic Engineering
Nanostructured Surfaces
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 18479804
- Database :
- OpenAIRE
- Journal :
- Nanomaterials and Nanotechnology
- Accession number :
- edsair.doi.dedup.....831edd593b3d8cb8ae41219253d95649