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Mucoadhesive liposomes for intranasal immunization with an avian influenza virus vaccine in chickens
- Source :
- Biomaterials. 30:5862-5868
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- The aim of this study was to characterize a nasally delivered bioadhesive liposome using an inactivated H5N3 virus as a model antigen. Bioadhesive liposomes were developed using tremella (T) or xanthan gum (XG) as the bioadhesive polysaccharide. Using chickens as the target animal, we evaluated whether delivery of a bioadhesive liposomal influenza vaccine via a mucosal site of infection could improve vaccine effectiveness. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cytotoxicity assays demonstrated that T, XG and liposomes were non toxic to chicken spleen macrophages. Enzyme-linked immunosorbent assay (ELISA) was used to determine the adjuvant effect of the bioadhesive liposomal-vaccines. Chickens immunized with a low dose (200 microL) of bioadhesive liposomal influenza vaccine had significantly higher mucosal and serum antibody levels (P
- Subjects :
- Influenza vaccine
Bioadhesive
medicine.medical_treatment
Biophysics
Biocompatible Materials
Bioengineering
Biology
medicine.disease_cause
Microbiology
Biomaterials
Antigen
Materials Testing
Influenza A virus
medicine
Animals
Administration, Intranasal
Liposome
Adhesiveness
Vaccination
Nasal Mucosa
Treatment Outcome
Influenza Vaccines
Mechanics of Materials
Influenza in Birds
Liposomes
Ceramics and Composites
Nasal administration
Chickens
Adjuvant
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....831ea64ee3464606e4df78f2fe34e746