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LINC00702 suppresses proliferation and invasion in non-small cell lung cancer through regulating miR-510/PTEN axis
- Source :
- Aging (Albany NY)
- Publication Year :
- 2019
- Publisher :
- Impact Journals, LLC, 2019.
-
Abstract
- Background Long non-coding RNAs (lncRNAs) have been consistently reported to be involved in the progression of non-small cell lung cancer (NSCLC). In this study, we aimed to identify aberrantly expressed lncRNAs in NSCLC, in order to explore new therapeutic targets for NSCLC. Methods Two pairs of NSCLC and adjacent normal tissues were first analyzed by RNA sequencing. The expressions of LINC00702 in 40 pairs patient samples and in 4 NSCLC cell lines was measured by quantitative real-time PCR. Putative target miRNAs of LINC00702 were predicted by the bioinformatics tools. The effect of LINC00702 on tumor growth in vivo was evaluated. Results LINC00702 was significantly down-regulated in patients with NSCLC, which was correlated with tumor size and metastasis. In addition, overexpression of LINC00702 markedly suppressed proliferation and metastasis in NSCLC cells via inducing apoptosis in vitro and in vivo. Moreover, bioinformatics and luciferase reporter assays demonstrated that LINC00702 functioned as a competing endogenous RNA (ceRNA) for miR-510 in NSCLC, and upregulated its target gene PTEN. Conclusion Our results indicated that LINC00702 modulated the expression of PTEN gene by acting as a ceRNA for miR-510 in NSCLC. Therefore, LINC00702 may serve as a potential target for the diagnosis and treatment of patients with NSCLC.
- Subjects :
- PTEN
Aging
Down-Regulation
Mice, Nude
Apoptosis
miR-510
Metastasis
Mice
Random Allocation
Downregulation and upregulation
Cell Movement
In vivo
Carcinoma, Non-Small-Cell Lung
Cell Line, Tumor
microRNA
medicine
Animals
Humans
Gene Silencing
Lung cancer
neoplasms
non-small cell lung cancer
Cell Proliferation
biology
Competing endogenous RNA
PTEN Phosphohydrolase
RNA
Neoplasms, Experimental
Cell Biology
medicine.disease
LINC00702
respiratory tract diseases
Gene Expression Regulation, Neoplastic
MicroRNAs
biology.protein
Cancer research
Female
RNA, Long Noncoding
Research Paper
Subjects
Details
- ISSN :
- 19454589
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Aging
- Accession number :
- edsair.doi.dedup.....830e4431aac77d152c4ec1d43c5791c6