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Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces
- Source :
- Cells, Volume 10, Issue 2, Cells, Vol 10, Iss 481, p 481 (2021)
- Publication Year :
- 2021
-
Abstract
- Current gold-standard strategies for bone regeneration do not achieve the optimal recovery of bone biomechanical properties. To bypass these limitations, tissue engineering techniques based on hybrid materials made up of osteoprogenitor cells—such as mesenchymal stem cells (MSCs)—and bioactive ceramic scaffolds—such as calcium phosphate-based (CaPs) bioceramics—seem promising. The biological properties of MSCs are influenced by the tissue source. This study aims to define the optimal MSC source and construct (i.e., the MSC–CaP combination) for clinical application in bone regeneration. A previous iTRAQ analysis generated the hypothesis that anatomical proximity to bone has a direct effect on MSC phenotype. MSCs were isolated from adipose tissue, bone marrow, and dental pulp, then cultured both on a plastic surface and on CaPs (hydroxyapatite and β-tricalcium phosphate), to compare their biological features. On plastic, MSCs isolated from dental pulp (DPSCs) presented the highest proliferation capacity and the greatest osteogenic potential. On both CaPs, DPSCs demonstrated the greatest capacity to colonise the bioceramics. Furthermore, the results demonstrated a trend that DPSCs had the most robust increase in ALP activity. Regarding CaPs, β-tricalcium phosphate obtained the best viability results, while hydroxyapatite had the highest ALP activity values. Therefore, we propose DPSCs as suitable MSCs for cell-based bone regeneration strategies.
- Subjects :
- 0301 basic medicine
Adult
Calcium Phosphates
Male
Bone Regeneration
Medicina
Cell Survival
Cell
regenerative medicine
Adipose tissue
Regenerative medicine
Article
03 medical and health sciences
0302 clinical medicine
stomatognathic system
Tissue engineering
Cirugía
Osteogenesis
Biological property
medicine
Humans
Bone regeneration
lcsh:QH301-705.5
mesenchymal stem cell
Biología celular
Chemistry
Mesenchymal stem cell
hydroxyapatite
Mesenchymal Stem Cells
030206 dentistry
General Medicine
Middle Aged
Alkaline Phosphatase
Genética
Cell biology
030104 developmental biology
medicine.anatomical_structure
Durapatite
lcsh:Biology (General)
Isotope Labeling
Female
Bone marrow
dental pulp
beta-tricalcium phosphate
Plastics
Subjects
Details
- ISSN :
- 20734409
- Volume :
- 10
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....82f10818ea286ec7a33f9a1b5f4cbeae