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Hyperoxia enhances expression of gamma-glutamyl transpeptidase and increases protein S-glutathiolation in rat lung
- Source :
- The American journal of physiology. 270(1 Pt 1)
- Publication Year :
- 1996
-
Abstract
- By participating in glutathione (GSH) synthesis, gamma-glutamyl transpeptidase (GGT) influences the GSH redox cycle, which is a major contributor in protecting against reactive oxygen metabolites. This study determined the effect of prolonged exposure of neonatal rats to > 98% oxygen on expression of GGT and on GSH metabolism. Lungs of neonatal rats chronically exposed to hyperoxia had increased expression of GGT mRNA, resulting in significantly higher GGT protein levels and enzyme activity than in lungs of animals raised in room air. Hyperoxia also upregulated glucose-6-phosphate dehydrogenase, but Na-K-ATPase activity was not changed. GGT mRNA, protein level, and enzyme activity returned to control levels after recovery in room air for 3 days. Levels of GSH, glutathione disulfide, and protein-bound GSH (S-glutathiolated protein) rose with hyperoxia and fell during recovery. S-glutathiolation is likely a mechanism for protection and a regulatory modification of protein sulfhydryl groups. Hyperoxia-induced upregulation of GGT and the concomitant increase in protein S-glutathiolation appear to be additional components fundamental in protecting the lung against oxidative injury.
- Subjects :
- Pulmonary and Respiratory Medicine
medicine.medical_specialty
Antioxidant
Physiology
medicine.medical_treatment
Blotting, Western
Biology
Glucosephosphate Dehydrogenase
Hyperoxia
medicine.disease_cause
digestive system
Protein S
Rats, Sprague-Dawley
chemistry.chemical_compound
Physiology (medical)
Internal medicine
medicine
Animals
RNA, Messenger
Lung
Cell Biology
Metabolism
Glutathione
gamma-Glutamyltransferase
Blotting, Northern
Immunohistochemistry
digestive system diseases
Enzyme assay
Rats
Endocrinology
chemistry
Biochemistry
Animals, Newborn
biology.protein
Glutathione disulfide
medicine.symptom
Oxidative stress
Subjects
Details
- ISSN :
- 00029513
- Volume :
- 270
- Issue :
- 1 Pt 1
- Database :
- OpenAIRE
- Journal :
- The American journal of physiology
- Accession number :
- edsair.doi.dedup.....82cdb7208ddbdc3339b6e7ad04360f2f