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Study on the effect of active components of Schisandra chinensis on liver injury and its mechanisms in mice based on network pharmacology
- Source :
- European Journal of Pharmacology. 910:174442
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The aim of this study was to analyze the active components of Schisandra chinensis on liver injury and its mechanism in mice by network pharmacology. The active components of S. chinensis were found through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and their corresponding targets were predicted. The targets of liver injury were searched through Therapeutic Targets Database (TTD), DisGeNET and drugbank databases, and the Venn diagram was constructed to obtain the action targets. The "drug-active component-target" network and protein-protein interaction network (PPI) were constructed by using STRING database and Cytoscape software, and the key targets were further screened by the enrichment analysis of relevant KEGG pathways. Finally, a CCl4-induced mouse liver injury model was established to verify the efficacy and related targets of S. chinensis and clarify its mechanism. Eight active components and 56 related targets of S. chinensis were screened out based on their oral bioavailability (OB) and drug likeness (DL). Five targets of S. chinensis related to liver injury were found by using the Venn diagram. The key targets, namely Ptgs2 and Nos2 genes, were further screened out by constructing a PPI network, and Schisandrol B (SCB) was considered the key component most closely related to the liver injury in S. chinensis. The results indicate that SCB may play a role in the treatment of the CCl4-induced liver injury by down-regulating the expression of iNOS and COX-2, and regulating the expression of NF-κB and IL-17 signaling pathway to inhibit the expression of proinflammatory factors.
- Subjects :
- Male
Schisandra chinensis
Nitric Oxide Synthase Type II
Computational biology
Network Pharmacology
Protective Agents
NF-KappaB Inhibitor alpha
Interaction network
medicine
Animals
Protein Interaction Maps
Medicine, Chinese Traditional
KEGG
Carbon Tetrachloride
Schisandra
TNF Receptor-Associated Factor 6
Pharmacology
Liver injury
Mice, Inbred ICR
biology
Mechanism (biology)
Interleukin-17
NF-kappa B
Transcription Factor RelA
biology.organism_classification
medicine.disease
Disease Models, Animal
Liver
Cyclooxygenase 2
Chemical and Drug Induced Liver Injury
Signal transduction
DrugBank
Drugs, Chinese Herbal
Signal Transduction
Systems pharmacology
Subjects
Details
- ISSN :
- 00142999
- Volume :
- 910
- Database :
- OpenAIRE
- Journal :
- European Journal of Pharmacology
- Accession number :
- edsair.doi.dedup.....82cc16be9aac54dab986003305391918