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Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome
- Source :
- Genes & Development. 22:2359-2369
- Publication Year :
- 2008
- Publisher :
- Cold Spring Harbor Laboratory, 2008.
-
Abstract
- Promoter recognition by TATA-binding protein (TBP) is an essential step in the initiation of RNA polymerase II (pol II) mediated transcription. Genetic and biochemical studies in yeast have shown that Mot1p and NC2 play important roles in inhibiting TBP activity. To understand how TBP activity is regulated in a genome-wide manner, we profiled the binding of TBP, NC2, Mot1p, TFIID, SAGA, and pol II across the yeast genome using chromatin immunoprecipitation (ChIP)–chip for cells in exponential growth and during reprogramming of transcription. We find that TBP, NC2, and Mot1p colocalize at transcriptionally active pol II core promoters. Relative binding of NC2α and Mot1p is higher at TATA promoters, whereas NC2β has a preference for TATA-less promoters. In line with the ChIP–chip data, we isolated a stable TBP–NC2–Mot1p–DNA complex from chromatin extracts. ATP hydrolysis releases NC2 and DNA from the Mot1p–TBP complex. In vivo experiments indicate that promoter dissociation of TBP and NC2 is highly dynamic, which is dependent on Mot1p function. Based on these results, we propose that NC2 and Mot1p cooperate to dynamically restrict TBP activity on transcribed promoters.
- Subjects :
- Saccharomyces cerevisiae Proteins
genetic processes
information science
Cell Cycle Proteins
RNA polymerase II
Saccharomyces cerevisiae
macromolecular substances
Transcription (biology)
Gene Expression Regulation, Fungal
Genetics
Promoter Regions, Genetic
Adenosine Triphosphatases
TATA-Binding Protein Associated Factors
biology
TATA-Box Binding Protein
DNA Helicases
Promoter
Molecular biology
Cell biology
enzymes and coenzymes (carbohydrates)
Trans-Activators
health occupations
biology.protein
RNA Polymerase II
Genome, Fungal
Transcription factor II D
TATA-binding protein
Chromatin immunoprecipitation
Transcription factor II A
Research Paper
Developmental Biology
Subjects
Details
- ISSN :
- 15495477 and 08909369
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Genes & Development
- Accession number :
- edsair.doi.dedup.....82bfb369a701b6685364dd3ad20c9511