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Retrotransposon-based insertion polymorphisms (RBIP) for high throughput marker analysis
- Source :
- The Plant journal : for cell and molecular biology. 16(5)
- Publication Year :
- 1999
-
Abstract
- Summary Two assays based upon PCR detection of a polymorphic PDR1 retrotransposon insertion in Pisum sativum have been developed. Both methods involve PCR with primers derived from the transposon and flanking DNA. The first method uses a dot assay for PCR product detection which could be fully automated for handling thousands of samples. The second method, which is designed to handle lower numbers, requires a single PCR and gel lane per sample. Both methods yield co-dominant markers, with presence and absence of the transposon insertion independently scorable, and both could in principle be applied to any transposable element in any plant species.
- Subjects :
- Transposable element
Genetic Markers
DNA, Plant
Retroelements
Pcr cloning
Molecular Sequence Data
Retrotransposon
Plant Science
Marker analysis
Biology
Polymerase Chain Reaction
law.invention
chemistry.chemical_compound
law
Genetics
Polymerase chain reaction
Alleles
DNA Primers
Polymorphism, Genetic
Base Sequence
Peas
food and beverages
Cell Biology
chemistry
Genetic marker
Plant species
DNA
Subjects
Details
- ISSN :
- 09607412
- Volume :
- 16
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biology
- Accession number :
- edsair.doi.dedup.....82bc1490155b6d010f6014d7a1a2496e