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CircC3P1 attenuated pro‐inflammatory cytokine production and cell apoptosis in acute lung injury induced by sepsis through modulating miR‐21
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2020
- Publisher :
- John Wiley and Sons Inc., 2020.
-
Abstract
- Acute lung injury (ALI) induced by sepsis is characterized by an inflammatory process related to the up‐regulation of inflammatory cytokines and chemokines. In the present study, we explored the role of circC3P1 in sepsis‐induced ALI in vitro and in vivo. The caecal ligation and puncture (CLP)‐induced sepsis model was established through CLP surgery. Forty adult male C57BL/6 mice were randomly assigned into sham, CLP, CLP + vector and CLP + circC3P1 (each n = 10). Primary murine pulmonary microvascular endothelial cells (MPVECs) were transfected with circC3P1 or empty vector 24 hours prior to LPS treatment via Lipofectamine 2000. The expressions of circC3P1, tumour necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6) and IL‐1β were evaluated after 6‐h LPS treatment. Cell apoptosis was evaluated via flow cytometry. The CLP group demonstrated pulmonary morphological abnormalities, increased concentrations of TNF‐α, IL‐6 and IL‐1β in the lung tissue, compared with the sham group. MPVECs treated with LPS significantly elevated TNF‐α, IL‐6 and IL‐1β levels and increased cell apoptosis than that in the control group. The circC3P1 overexpression in sepsis‐induced ALI mice attenuated pulmonary injury, inflammation and apoptosis. Besides, circC3P1 revealed anti‐inflammatory and anti‐apoptotic effect in MPVEC‐treated LPS. CircC3P1 overexpression reduced cell apoptosis and pro‐inflammatory cytokines levels via down‐regulating miR‐21. CircC3P1 attenuated pro‐inflammatory cytokine production and cell apoptosis in ALI induced by sepsis through modulating miR‐21, indicating that circC3P1 is a promising therapeutic biomarker for sepsis‐induced ALI.
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
Male
Chemokine
Necrosis
medicine.medical_treatment
Acute Lung Injury
Down-Regulation
Inflammation
Apoptosis
Punctures
Pharmacology
Lung injury
Proinflammatory cytokine
Sepsis
sepsis
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Phosphorylation
Cecum
Ligation
Lung
circC3P1
biology
business.industry
Endothelial Cells
Cell Biology
Original Articles
RNA, Circular
medicine.disease
Mice, Inbred C57BL
MicroRNAs
030104 developmental biology
Cytokine
030220 oncology & carcinogenesis
Microvessels
biology.protein
Molecular Medicine
Cytokines
Original Article
medicine.symptom
Inflammation Mediators
business
Proto-Oncogene Proteins c-akt
Subjects
Details
- Language :
- English
- ISSN :
- 15824934 and 15821838
- Volume :
- 24
- Issue :
- 19
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....82a413d46a91875896524a6ec8e3dfca