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Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice
- Source :
- Proceedings of the National Academy of Sciences. 92:4857-4861
- Publication Year :
- 1995
- Publisher :
- Proceedings of the National Academy of Sciences, 1995.
-
Abstract
- Cells from transgenic mice expressing a human mini-gene for collagen I were used as markers to follow the fate of mesenchymal precursor cells from marrow that were partially enriched by adherence to plastic, expanded in culture, and then injected into irradiated mice. Sensitive PCR assays for the marker collagen I gene indicated that few of the donor cells were present in the recipient mice after 1 week, but 1-5 months later, the donor cells accounted for 1.5-12% of the cells in bone, cartilage, and lung in addition to marrow and spleen. A PCR in situ assay on lung indicated that the donor cells diffusely populated the parenchyma, and reverse transcription-PCR assays indicated that the marker collagen I gene was expressed in a tissue-specific manner. The results, therefore, demonstrated that mesenchymal precursor cells from marrow that are expanded in culture can serve as long-lasting precursors for mesenchymal cells in bone, cartilage, and lung. They suggest that cells may be particularly attractive targets for gene therapy ex vivo.
- Subjects :
- Genetic enhancement
Cellular differentiation
Molecular Sequence Data
Gene Expression
Bone Marrow Cells
Mice, Inbred Strains
Mice, Transgenic
Spleen
Biology
Polymerase Chain Reaction
Bone and Bones
Mice
Cell Adhesion
medicine
Animals
Humans
Lung
Cells, Cultured
Bone Marrow Transplantation
DNA Primers
Stem cell transplantation for articular cartilage repair
Multidisciplinary
Base Sequence
Stem Cells
Cartilage
Mesenchymal stem cell
Cell Differentiation
Molecular biology
medicine.anatomical_structure
Cesium Radioisotopes
Collagen
Stem cell
Ex vivo
Research Article
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....829ef9ddbffd1fb51c213c163a209922