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Paper-based bioactive scaffolds for stem cell-mediated bone tissue engineering
- Source :
- Biomaterials. 35:9811-9823
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Bioactive, functional scaffolds are required to improve the regenerative potential of stem cells for tissue reconstruction and functional recovery of damaged tissues. Here, we report a paper-based bioactive scaffold platform for stem cell culture and transplantation for bone reconstruction. The paper scaffolds are surface-engineered by an initiated chemical vapor deposition process for serial coating of a water-repellent and cell-adhesive polymer film, which ensures the long-term stability in cell culture medium and induces efficient cell attachment. The prepared paper scaffolds are compatible with general stem cell culture and manipulation techniques. An optimal paper type is found to provide structural, physical, and mechanical cues to enhance the osteogenic differentiation of human adipose-derived stem cells (hADSCs). A bioactive paper scaffold significantly enhances in vivo bone regeneration of hADSCs in a critical-sized calvarial bone defect. Stacking the paper scaffolds with osteogenically differentiated hADSCs and human endothelial cells resulted in vascularized bone formation in vivo. Our study suggests that paper possesses great potential as a bioactive, functional, and cost-effective scaffold platform for stem cell-mediated bone tissue engineering. To the best of our knowledge, this is the first study reporting the feasibility of a paper material for stem cell application to repair tissue defects.
- Subjects :
- Paper
Scaffold
Bone Regeneration
Materials science
Cell
Cell Culture Techniques
Biophysics
Mice, Nude
Bioengineering
Gene delivery
Biomaterials
Extracellular matrix
Mice
Osteogenesis
medicine
Animals
Humans
Bone regeneration
Cells, Cultured
Mice, Inbred BALB C
Tissue Engineering
Tissue Scaffolds
Stem Cells
Skull
Cell Differentiation
Transplantation
medicine.anatomical_structure
Adipose Tissue
Mechanics of Materials
Ceramics and Composites
Stem cell
Bioactive paper
Stem Cell Transplantation
Biomedical engineering
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....827a84bea2832f109fb9e3be27e921d3