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A Lipid Photoswitch Controls Fluidity in Supported Bilayer Membranes
- Source :
- Langmuir : the ACS journal of surfaces and colloids. 36(10)
- Publication Year :
- 2020
-
Abstract
- Supported lipid bilayer (SLB) membranes are key elements to mimic membrane interfaces on a planar surface. Here, we demonstrate that azobenzene photolipids (azo-PC) form fluid, homogeneous SLBs. Diffusion properties of azo-PC within SLBs were probed by fluorescence microscopy and fluorescence recovery after photobleaching. At ambient conditions, we find that the trans-to-cis isomerization causes an increase of the diffusion constant by a factor of two. Simultaneous excitation with two wavelengths and variable intensities furthermore allows to adjust the diffusion constant D continuously. X-ray reflectometry and small-angle scattering measurements reveal that membrane photoisomerization results in a bilayer thickness reduction of ∼0.4 nm (or 10%). While thermally induced back-switching is not observed, we find that the trans bilayer fluidity is increasing with higher temperatures. This change in diffusion constant is accompanied by a red-shift in the absorption spectra. Based on these results, we suggest that the reduced diffusivity of trans-azo-PC is controlled by intermolecular interactions that also give rise to H-aggregate formation in bilayer membranes.
- Subjects :
- Materials science
Photoswitch
Photoisomerization
Diffusion
Bilayer
Fluorescence recovery after photobleaching
02 engineering and technology
Surfaces and Interfaces
010402 general chemistry
021001 nanoscience & nanotechnology
Condensed Matter Physics
01 natural sciences
Fick's laws of diffusion
0104 chemical sciences
Membrane
Chemical physics
Electrochemistry
General Materials Science
0210 nano-technology
Lipid bilayer
Spectroscopy
Subjects
Details
- ISSN :
- 15205827
- Volume :
- 36
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Langmuir : the ACS journal of surfaces and colloids
- Accession number :
- edsair.doi.dedup.....82734a47b398d615cd6c9d024a3ea630