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11β-hydroxysteroid dehydrogenase type-2 and type-1 (11β-HSD2 and 11β-HSD1) and 5β-reductase activities in the pathogenia of essential hypertension
- Source :
- ENDOCRINE (BASINGSTOKE), Artículos CONICYT, CONICYT Chile, instacron:CONICYT
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Cortisol availability is modulated by several enzymes: 11β-HSD2, which transforms cortisol (F) to cortisone (E) and 11β-HSD1 which predominantly converts inactive E to active F. Additionally, the A-ring reductases (5α- and 5β-reductase) inactivate cortisol (together with 3α-HSD) to tetrahydrometabolites: 5αTHF, 5βTHF, and THE. The aim was to assess 11β-HSD2, 11β-HSD1, and 5β-reductase activity in hypertensive patients. Free urinary F, E, THF, and THE were measured by HPLC-MS/MS in 102 essential hypertensive patients and 18 normotensive controls. 11β-HSD2 enzyme activity was estimated by the F/E ratio, the activity of 11β-HSD1 in compare to 11β-HSD2 was inferred by the (5αTHF + 5βTHF)/THE ratio and 5β-reductase activity assessed using the E/THE ratio. Activity was considered altered when respective ratios exceeded the maximum value observed in the normotensive controls. A 15.7% of patients presented high F/E ratio suggesting a deficit of 11β-HSD2 activity. Of the remaining 86 hypertensive patients, two possessed high (5αTHF + 5βTHF)/THE ratios and 12.8% had high E/THE ratios. We observed a high percentage of alterations in cortisol metabolism at pre-receptor level in hypertensive patients, previously misclassified as essential. 11β-HSD2 and 5β-reductase decreased activity and imbalance of 11β-HSDs should be considered in the future management of hypertensive patients.
- Subjects :
- Adult
Male
medicine.medical_specialty
Hydrocortisone
Endocrinology, Diabetes and Metabolism
Urinary system
5β reductase
11β hsd2
Essential hypertension
Endocrinology
Tandem Mass Spectrometry
11-beta-Hydroxysteroid Dehydrogenase Type 2
Internal medicine
Diabetes mellitus
hypertension
11beta-hydroxysteroid
5beta-reductase
11-beta-Hydroxysteroid Dehydrogenase Type 1
medicine
Humans
Chile
Tetrahydrocortisol
Chromatography, High Pressure Liquid
17-Hydroxycorticosteroids
chemistry.chemical_classification
biology
Chemistry
Mineralocorticoid Excess Syndrome, Apparent
Middle Aged
3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
medicine.disease
Enzyme assay
Cortisone
Enzyme
Tetrahydrocortisone
Hypertension
biology.protein
Female
Oxidoreductases
Algorithms
medicine.drug
Subjects
Details
- ISSN :
- 15590100 and 1355008X
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Endocrine
- Accession number :
- edsair.doi.dedup.....8265ab87e2e1f1f7385a487543b148a3