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In Vitro Induction and In Vivo Engraftment of Lung Bud Tip Progenitor Cells Derived from Human Pluripotent Stem Cells
- Source :
- Stem Cell Reports, Vol 10, Iss 1, Pp 101-119 (2018), Stem Cell Reports
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- Summary The current study aimed to understand the developmental mechanisms regulating bud tip progenitor cells in the human fetal lung, which are present during branching morphogenesis, and to use this information to induce a bud tip progenitor-like population from human pluripotent stem cells (hPSCs) in vitro. We identified cues that maintained isolated human fetal lung epithelial bud tip progenitor cells in vitro and induced three-dimensional hPSC-derived organoids with bud tip-like domains. Bud tip-like domains could be isolated, expanded, and maintained as a nearly homogeneous population. Molecular and cellular comparisons revealed that hPSC-derived bud tip-like cells are highly similar to native lung bud tip progenitors. hPSC-derived epithelial bud tip-like structures survived in vitro for over 16 weeks, could be easily frozen and thawed, maintained multilineage potential, and successfully engrafted into the airways of immunocompromised mouse lungs, where they persisted for up to 6 weeks and gave rise to several lung epithelial lineages.<br />Graphical Abstract<br />Highlights • Fgf7, CHIR-99021, and RA support growth of human lung bud tip progenitors in vitro • These factors induce lung bud tip progenitor-like organoids from hPSCs in vitro • hPSC-derived organoids are highly similar to human fetal bud tip progenitors • hPSC-derived bud tip organoids engraft into the injured murine airway<br />In this article, Jason Spence and colleagues identify a core signaling network that maintains isolated human epithelial lung bud tip progenitors in vitro and that induces a bud tip-like population from hPSCs. They show that iPSC-derived bud tip progenitors can engraft into an injured mouse airway, contribute to re-epithelialization, and differentiate into multiple lung epithelial lineages.
- Subjects :
- 0301 basic medicine
Pluripotent Stem Cells
organoid
lung organoid
Population
SOX2
Mice, Transgenic
Lung bud
Biology
Biochemistry
Article
lung
03 medical and health sciences
Mice
Directed differentiation
Fetus
Genetics
Organoid
Animals
Humans
human pluripotent stem cell
Progenitor cell
Induced pluripotent stem cell
education
lcsh:QH301-705.5
Progenitor
education.field_of_study
lcsh:R5-920
directed differentiation
progenitor
Cell Biology
bud tip
3. Good health
Cell biology
030104 developmental biology
lcsh:Biology (General)
Heterografts
branching morphogenesis
lcsh:Medicine (General)
SOX9
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 22136711
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Stem Cell Reports
- Accession number :
- edsair.doi.dedup.....825da62ecb6d556e97ce0bfda9536c7b