Back to Search
Start Over
Benzene metabolite, 1,2,4-benzenetriol, induces micronuclei and oxidative DNA damage in human lymphocytes and HL60 cells
- Source :
- Environmental and molecular mutagenesis. 21(4)
- Publication Year :
- 1993
-
Abstract
- The triphenolic metabolite of benzene, 1,2,4-benzenetriol (BT), is readily oxidized to its corresponding quinone via a semiquinone radical. During this process, active oxygen species are formed that may damage DNA and other cellular macromolecules. The ability of BT to induce micronuclei (MN) and oxidative DNA damage has been investigated in both human lymphocytes and HL60 cells. An antikinetochore antibody based micronucleus assay was used to distinguish MN containing kinetochores and potentially entire chromosomes (kinetochore-positive, K+) from those containing acentric chromosome fragments (kinetochore-negative, K−). BT increased the frequency of MN formation twofold in lymphocytes and eightfold in HL60 cells with the MN being 62% and 82% K+, respectively. A linear dose-related increase in total MN, mainly in K+-MN, was observed in both HL60 cells and lymphocytes. Addition of copper ions (Cu2+) potentiated the effect of BT on MN induction threefold in HL60 cells and altered the pattern of MN formation from predominantly K+ to K−. BT also increased the level of 8-hydroxy-2′-deoxyguanosine (8-OH-dG), a marker of active oxygen-induced DNA damage. Cu2+ again enhanced this effect. Thus, BT has the potential to cause both numerical and structural chromosomal changes in human cells. Further, it may cause point mutations indirectly by generating oxygen radicals. BT may therefore play an important role in benzene-induced leukemia. © 1993 Wiley-Liss, Inc.
- Subjects :
- Benzenetriol
Adult
Semiquinone
Free Radicals
Epidemiology
DNA damage
Health, Toxicology and Mutagenesis
Lymphocyte
Metabolite
Centromere
chemistry.chemical_compound
medicine
Tumor Cells, Cultured
Humans
Lymphocytes
Genetics (clinical)
Analysis of Variance
Micronucleus Tests
8-Hydroxy-2'-deoxyguanosine
Deoxyguanosine
Drug Synergism
DNA, Neoplasm
Aneuploidy
Molecular biology
Hydroquinones
medicine.anatomical_structure
Cell Transformation, Neoplastic
chemistry
Biochemistry
8-Hydroxy-2'-Deoxyguanosine
Leukemia, Myeloid
Micronucleus test
Regression Analysis
Female
Micronucleus
Reactive Oxygen Species
Copper
DNA Damage
Mutagens
Subjects
Details
- ISSN :
- 08936692
- Volume :
- 21
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Environmental and molecular mutagenesis
- Accession number :
- edsair.doi.dedup.....824c39f18a012585e1433d8c0423e5f7