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Peanut Agglutinin High Phenotype of Activated CD8+ T Cells Results from de Novo Synthesis of CD45 Glycans
- Source :
- Journal of Biological Chemistry. 279:36689-36697
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Following activation in the periphery, murine CD8+ T cells exhibit a characteristic increased binding of peanut agglutinin (PNA), reflecting an increased expression of hyposialylated O-linked glycans (Galbeta1-3GalNAcalpha-O-Thr/Ser) on the cell surface. In this report, we show that the majority of the PNA receptors expressed on activated CD8+ T cells are carried by CD45. Other glycoproteins (e.g. CD8) and the glycolipid asialo-GM1 also carry PNA receptors, although to a much lesser extent. Analysis of enzymes involved in the sialylation/de-sialylation pathways showed that generation of PNA receptors in activated CD8+ T cells is not due to up-regulation of endogenous sialidases. Instead, our results indicate that the PNA(high) phenotype results from de novo synthesis of CD45 carrying reduced sialylated core 1 O-glycans.
- Subjects :
- Peanut agglutinin
Glycan
beta-Galactoside alpha-2,3-Sialyltransferase
T-Lymphocytes
Blotting, Western
Biotin
Neuraminidase
CD8-Positive T-Lymphocytes
Sialidase
Models, Biological
Biochemistry
Mice
Peanut Agglutinin
Glycolipid
Polysaccharides
Lectins
Animals
Cytotoxic T cell
Receptor
Molecular Biology
Glycoproteins
chemistry.chemical_classification
biology
Reverse Transcriptase Polymerase Chain Reaction
Cell Membrane
Cell Biology
Flow Cytometry
Precipitin Tests
Molecular biology
Sialyltransferases
Mice, Inbred C57BL
carbohydrates (lipids)
Phenotype
Models, Chemical
chemistry
COS Cells
cardiovascular system
biology.protein
Leukocyte Common Antigens
RNA
Chromatography, Thin Layer
Glycoprotein
Spleen
CD8
Protein Binding
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 279
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....824a4db6d9ed04b353adc67a2c043664