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MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice
- Source :
- The Plant Cell. 28:1879-1893
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- F-box proteins constitute a large superfamily in plants and play important roles in controlling many biological processes, but the roles of F-box proteins in male meiosis in plants remain unclear. Here, we identify the rice (Oryza sativa) F-box gene MEIOTIC F-BOX (MOF), which is essential for male meiotic progression. MOF belongs to the FBX subfamily and is predominantly active during leptotene to pachytene of prophase I. mof meiocytes display disrupted telomere bouquet formation, impaired pairing and synapsis of homologous chromosomes, and arrested meiocytes at late prophase I, followed by apoptosis. Although normal, programmed double-stranded DNA breaks (DSBs) form in mof mutants, foci of the phosphorylated histone variant γH2AX, a marker for DSBs, persist in the mutant, indicating that many of the DSBs remained unrepaired. The recruitment of Completion of meiosis I (COM1) and Radiation sensitive51C (RAD51C) to DSBs is severely compromised in mutant meiocytes, indicating that MOF is crucial for DSB end-processing and repair. Further analyses showed that MOF could physically interact with the rice SKP1-like Protein1 (OSK1), indicating that MOF functions as a component of the SCF E3 ligase to regulate meiotic progression in rice. Thus, this study reveals the essential role of an F-box protein in plant meiosis and provides helpful information for elucidating the roles of the ubiquitin proteasome system in plant meiotic progression.
- Subjects :
- 0301 basic medicine
DNA Repair
DNA repair
Cell Cycle Proteins
Plant Science
Meiocyte
F-box protein
03 medical and health sciences
Meiosis
Homologous chromosome
DNA Breaks, Double-Stranded
Research Articles
Plant Proteins
Genetics
biology
urogenital system
F-Box Proteins
fungi
Synapsis
food and beverages
Oryza
Cell Biology
Ubiquitin ligase
030104 developmental biology
Histone
biology.protein
Subjects
Details
- ISSN :
- 1532298X and 10404651
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- The Plant Cell
- Accession number :
- edsair.doi.dedup.....823f8d4d35e9e6e5450c02bc8c983dc0