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Characterization of Flagellotropic, Chi-Like Salmonella Phages Isolated from Thai Poultry Farms
- Source :
- Viruses, Vol 11, Iss 6, p 520 (2019), Viruses, Volume 11, Issue 6, Viruses, 11 (6)
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Despite a wealth of knowledge on Salmonella phages worldwide, little is known about poultry-associated Salmonella phages from Thailand. Here, we isolated 108 phages from Thai poultry farms that infect Salmonella enterica serovar Typhimurium. Phages STm101 and STm118 were identified as temperate Siphoviridae phages. Genome sequencing and analyses revealed these phages share approximately 96% nucleotide sequence similarity to phage SPN19, a member of the Chi-like virus genus. PCR amplification of the gene encoding capsid protein E of the Chi-like phage was positive for 50% of phage isolates, suggesting a predominance of this phage type among the sampled poultry farms. In addition to the flagella, two phages required the lipopolysaccharide to infect and lyse Salmonella. Furthermore, phylogenomic analysis demonstrated that phages STm101 and STm118 formed a monophyletic clade with phages isolated from Western countries, but not from closer isolated phages from Korea. However, further investigation and more phage isolates are required to investigate possible causes for this geographic distribution.<br />Viruses, 11 (6)<br />ISSN:1999-4915
- Subjects :
- 0301 basic medicine
Serotype
prevalent
Salmonella
viruses
poultry farm
030106 microbiology
lcsh:QR1-502
medicine.disease_cause
bacteriophage
Chi-like virus
flagellotropic
siphovirus
phylogenomic tree
DNA sequencing
Virus
lcsh:Microbiology
law.invention
Microbiology
Siphoviridae
Bacteriophage
03 medical and health sciences
law
Virology
medicine
Polymerase chain reaction
biology
biology.organism_classification
030104 developmental biology
Infectious Diseases
Salmonella enterica
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 11
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....823c03fa9e5497745cd9e8e0ab8161a5