Back to Search
Start Over
Phosphatidylcholine protects neurons from toxic effects of amyloid β-protein in culture
- Source :
- Brain Research. 1642:376-383
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Amyloid β-protein (Aβ) is the major component of extracellular plaques in the brains of patients with Alzheimer's disease. It has been suggested that the interaction of Aβ with membrane cholesterol is essential for Aβ to exert neurotoxicity; however, the effect of phospholipids, another major membrane lipid component, on Aβ-induced neurotoxicity remains unclarified. Here we report the protective effect of phosphatidylcholine (PC) on primary cultured neurons against Aβ1-42-induced damage. Aβ1-42 caused neuronal death as demonstrated by lactose dehydrogenase (LDH) release, which was completely prevented by a pretreatment with PC in a dose-dependent manner. PC containing unsaturated long-chain acyl groups, 1,2-dioleoyl-PC (DOPC), also prevented neuronal death caused by Aβ1-42. The oleic acid ethyl-ester (OAEE) partially prevented Aβ1-42-induced neurotoxicity. Neurons that were pretreated with DOPC or OAEE for 24h, washed out, and exposed to Aβ1-42 in the absence of either of these reagents, were still resistant to Aβ1-42-induced neurotoxicity. In contrast, treatment with phosphotidylserine (PS) or docosahexaenoic acid etyl-ester (DHAEE) had no protective effect on neurons against Aβ1-42-induced damage. These results suggest that the control of cellular PC content, not PS content, may prove useful in the prevention or treatment of Alzheimer's disease.
- Subjects :
- 0301 basic medicine
Amyloid
Cell Survival
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Phosphatidylcholine
Extracellular
medicine
Animals
Lactate Dehydrogenases
Molecular Biology
Cells, Cultured
Cerebral Cortex
Neurons
Amyloid beta-Peptides
Dose-Response Relationship, Drug
Cholesterol
General Neuroscience
Neurotoxicity
Phosphatidylserine
medicine.disease
Peptide Fragments
Rats
Oleic acid
Neuroprotective Agents
030104 developmental biology
chemistry
Biochemistry
Docosahexaenoic acid
Phosphatidylcholines
Neurology (clinical)
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1642
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....823aa953fd472b241e2cfac64380130a