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PRAME expression in melanocytic lesions of the conjunctiva
- Source :
- Histopathology. 79:989-996
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- AIMS PRAME (PReferentially expressed Antigen in MElanoma) is a tumour-associated antigen that is preferentially strongly expressed in most cutaneous melanomas but not or only focally in naevi. Our aim was to evaluate PRAME expression in melanocytic lesions of the conjunctiva. METHODS AND RESULTS Surgical specimens of 114 conjunctival melanocytic naevi of different types (including 67 common, 25 combined deep penetrating and 21 inflamed juvenile naevi), 30 invasive melanomas, 10 in-situ melanomas, 23 primary acquired melanoses (PAM) without atypia and 11 PAM with atypia were analysed for PRAME expression by immunohistochemistry. Nuclear positivity for PRAME in melanocytes was assessed as the percentage of positive nuclei: negative (0%), 1+ (1-25%), 2+ (26-50%), 3+ (51-75%) and 4+ (> 75%). In 113 of 114 conjunctival melanocytic naevi, PRAME was either completely negative or focally 1+ positive. Diffuse 4+ PRAME expression was identified in 17 of 30 (57%) invasive melanomas, seven of 10 (70%) in-situ melanomas, four of five (80%) PAM with severe atypia, none of three PAM with moderate atypia, none of three PAM with mild atypia, one of 23 (4%) PAM without atypia and none of 114 naevi. Diffuse 4+ PRAME expression in invasive melanomas correlated with a higher mitotic count but was not related to age and gender of the patients, Breslow thickness, location or mutational status. CONCLUSION Diffuse 4+ PRAME positivity is highly specific for malignant conjunctival melanocytic lesions. PRAME is therefore a useful ancillary marker to support the diagnosis of a suspected conjunctival melanoma.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Histology
Conjunctiva
Adolescent
Conjunctival Neoplasms
Pathology and Forensic Medicine
Young Adult
Antigens, Neoplasm
Biomarkers, Tumor
Atypia
Humans
Medicine
Nevus
Child
skin and connective tissue diseases
Melanoma
neoplasms
Aged
Aged, 80 and over
PRAME
business.industry
General Medicine
Middle Aged
medicine.disease
Melanosis
medicine.anatomical_structure
Child, Preschool
Immunohistochemistry
Female
business
Conjunctival Melanoma
Subjects
Details
- ISSN :
- 13652559 and 03090167
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Histopathology
- Accession number :
- edsair.doi.dedup.....821864c5e43bc993475d20f68c4bf9d5