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Comparative evaluation of three dengue duo rapid test kits to detect NS1, IgM, and IgG associated with acute dengue in children in Myanmar
- Source :
- PLoS ONE, Vol 14, Iss 3, p e0213451 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Dengue is an increasing public health concern worldwide and requires efficient laboratory diagnostics. We evaluated three commercially available dengue rapid diagnostic tests—the Humasis Dengue Combo NS1 & IgG/IgM (Humasis, Korea), SD Bioline Dengue Duo NS1 Ag & IgG/IgM (SD Bioline, Korea), and CareUS Dengue Combo NS1 and IgM/IgG kits (WellsBio, Korea)—and compared them to reference immunoglobulin M (IgM) or immunoglobulin G (IgG) ELISAs and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. In total, 109 dengue-positive samples from children with acute symptomatic dengue and 63 dengue-negative samples from febrile and asymptomatic individuals were collected. For the nonstructural 1 protein (NS1) Ag test, the sensitivity and specificity were in the following order: CareUS (79.82 and 100%), Humasis (63.30 and 100%), and SD Bioline (48.62 and 100%). For IgM and IgG, CareUS had the highest sensitivities and specificities (89.91 and 100%; 82.57 and 100%, respectively), followed by SD Bioline (60.55 and 100%, 77.98 and 100%, respectively), and Humasis (51.38 and 98.21%, 72.48 and 95.24%, respectively). The IgM kits were more sensitive than the NS1 Ag or IgG kits; however, combining NS1 Ag and IgM reduced the number of missed cases. Therefore, the NS1 Ag plus IgM dengue kits increase the accuracy of the results. In our study, the CareUS Dengue Combo NS1 and IgM/IgG kit showed higher accuracy in performance with reference to qRT-PCR and ELISA results.
- Subjects :
- RNA viruses
Male
Viral Diseases
Physiology
Artificial Gene Amplification and Extension
Myanmar
Viral Nonstructural Proteins
Pathology and Laboratory Medicine
Antibodies, Viral
Biochemistry
Polymerase Chain Reaction
Immunoglobulin G
Dengue Fever
Dengue fever
Geographical Locations
Dengue
0302 clinical medicine
Immune Physiology
Medicine and Health Sciences
030212 general & internal medicine
Enzyme-Linked Immunoassays
Child
Cross Reactivity
Immune System Proteins
Multidisciplinary
biology
Infectious Diseases
Medical Microbiology
Viral Pathogens
Child, Preschool
Viruses
Medicine
Female
Pathogens
Antibody
Research Article
Neglected Tropical Diseases
Asia
Adolescent
Science
Immunology
030231 tropical medicine
Research and Analysis Methods
Microbiology
Antibodies
Comparative evaluation
03 medical and health sciences
medicine
Humans
Immunoassays
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Biology and life sciences
Flaviviruses
business.industry
Organisms
Proteins
Chikungunya Infection
Infant
Reverse Transcriptase-Polymerase Chain Reaction
Dengue Virus
Tropical Diseases
medicine.disease
Virology
Immunoglobulin M
People and Places
Immunologic Techniques
biology.protein
Reagent Kits, Diagnostic
business
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....81fc440f31428ec5576f14b0896b4d4b