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Isolation and Purification of Bacillus amyloliquefaciens D1 Protease and Its Application in the Fermentation of Soybean Milk to Produce Large Amounts of Free Amino Acids

Authors :
Liyu Du
Jie Wang
Weizhe Chen
Jujie Chen
Qikai Zheng
Xiang Fang
Zhenlin Liao
Source :
Applied Biochemistry and Biotechnology. 195:451-466
Publication Year :
2022
Publisher :
Springer Science and Business Media LLC, 2022.

Abstract

In this study, a strain of Bacillus amyloliquefaciens D1, with a notably high production of neutral protease, was isolated from Morchella crassipes. The protease was purified to 10.4-fold with a specific activity of 4542.9 U/mg and 2.7% recovery. The enzyme was purified by 70% (NH

Details

ISSN :
15590291 and 02732289
Volume :
195
Database :
OpenAIRE
Journal :
Applied Biochemistry and Biotechnology
Accession number :
edsair.doi.dedup.....81efaccb2c72b597e8659606018110a0
Full Text :
https://doi.org/10.1007/s12010-022-04133-x