Back to Search
Start Over
Effect of celiac ganglionectomy on tachykinin innervation, receptor distribution and intestinal responses in the rat
- Source :
- Journal of the Autonomic Nervous System. 61:292-300
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- Substance P (SP) is an important neurotransmitter in the control of intestinal motility and is found in both the enteric and sympathetic nervous systems. This study examined the effect of celiac ganglionectomy on (1) mechanical properties of the circular muscles of the duodenum, ileum and proximal colon, (2) circular muscle responses to SP and neurokinin A. (3) distribution of substance P-like immunoreactive nerves, and (4) the distribution of neurokinin 1 and neurokinin 2 receptors. Celiac ganglionectomy resulted in an effective sympathectomy as evidenced by a marked decrease in norepinephrine content and tyrosine hydroxylase staining in the duodenum, ileum and proximal colon. The in vitro length/tension characteristics of the circular muscle of the duodenum, ileum and colon were unchanged after ganglionectomy. In all regions of the gut studied, substance P and neurokinin A caused dose-dependent contractions that were unaltered by celiac ganglionectomy. Immunohistochemistry revealed moderate substance P-like immunoreactive fibers in the myenteric plexus, submucosal plexus and circular muscle of the ileum, while in the colon, substance P-like immunoreactivity was intense in the myenteric plexus, and moderate in the circular muscle. In vitro autoradiography showed minimal binding of SP (NK1 receptor) or neurokinin A (NK2 receptor) in the ileum and significantly greater binding in the circular muscle layer of the colon. Celiac ganglionectomy did not affect substance P-like immunoreactivity, or NK1 or NK2 receptor binding. A greater contractile response to neurokinins was seen in the colon than in the duodenum or ileum, which paralleled the receptor density. The studies demonstrate that surgical celiac ganglionectomy, unlike chemical sympathectomy, does not affect the substance P innervation, receptor density or physiological responses of the intestine. The greater contractile response of the colon than the ileum parallels the greater receptor density rather than the peptide content as determined by immunhistochemistry.
- Subjects :
- Male
medicine.medical_specialty
Physiology
Neurokinin A
medicine.medical_treatment
Substance P
Ileum
In Vitro Techniques
Biology
digestive system
Rats, Sprague-Dawley
Norepinephrine
chemistry.chemical_compound
Tachykinins
Internal medicine
Tachykinin receptor 1
medicine
Animals
Ganglionectomy
Intestinal Mucosa
Receptor
Receptors, Tachykinin
Myenteric plexus
Ganglia, Sympathetic
General Neuroscience
digestive, oral, and skin physiology
Muscle, Smooth
Immunohistochemistry
digestive system diseases
Rats
Intestines
medicine.anatomical_structure
Endocrinology
chemistry
Duodenum
Neurology (clinical)
Muscle Contraction
Subjects
Details
- ISSN :
- 01651838
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Journal of the Autonomic Nervous System
- Accession number :
- edsair.doi.dedup.....81e202afb2ca46f3d34196d6c8c13fb3