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Nα-acetyltransferase NatA is involved in ribosome synthesis in Saccharomyces cerevisiae
- Source :
- Bioscience, biotechnology, and biochemistry. 77(3)
- Publication Year :
- 2013
-
Abstract
- Ebp2 has an essential role in the biogenesis of 60S ribosomal subunits. Synthetic-sick alleles with the ebp2-14 mutation were screened. The mutations were localized to the ARD1 and NAT1 genes, which encode the catalytic subunit and the auxiliary subunit of N(α)-acetyltransferase NatA respectively. Polysome analyses revealed that ard1Δ and nat1Δ caused a synergistic defect with ebp2-14 in the assembly of 60S ribosomal subunits. To identify the proteins that functionally interact with NatA, we designed mutants in which the second amino acid was substituted for proline in Ebp2 and functionally related proteins: Brx1, a partner of Ebp2 in ribosome biogenesis, and the ribosomal protein L36a/b, overexpression of which suppresses a growth defect in ebp2-14. Among these, only brx1-S2P exhibited a synthetic defect with ebp2-14. These results suggest that optimal NatA function is important to the cooperative function of Brx1 with Ebp2 in 60S ribosomal subunit biogenesis.
- Subjects :
- Saccharomyces cerevisiae Proteins
Molecular Sequence Data
Ribosome biogenesis
Saccharomyces cerevisiae
Biology
Applied Microbiology and Biotechnology
Biochemistry
Ribosome
Analytical Chemistry
Ribosomal protein
Acetyltransferases
Animals
Humans
Eukaryotic Small Ribosomal Subunit
Amino Acid Sequence
Molecular Biology
Eukaryotic Large Ribosomal Subunit
Organic Chemistry
RNA-Binding Proteins
General Medicine
Ribosomal RNA
Ribosome Subunits, Large, Eukaryotic
Ribosome Subunits
Mutation
Eukaryotic Ribosome
Carrier Proteins
Biotechnology
Subjects
Details
- ISSN :
- 13476947
- Volume :
- 77
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Accession number :
- edsair.doi.dedup.....819bed742f1993066cda4e29741906e0