Back to Search
Start Over
Circulating MicroRNAs Associate With Diabetic Nephropathy and Systemic Microvascular Damage and Normalize After Simultaneous Pancreas-Kidney Transplantation
- Source :
- American Journal of Transplantation, 15(4), 1081-1090
- Publication Year :
- 2015
-
Abstract
- Because microvascular disease is one of the most important drivers of diabetic complications, early monitoring of microvascular integrity may be of clinical value. By assessing profiles of circulating microRNAs (miRNAs), known regulators of microvascular pathophysiology, in healthy controls and diabetic nephropathy (DN) patients before and after simultaneous pancreas-kidney transplantation (SPK), we aimed to identify differentially expressed miRNAs that associate with microvascular impairment. Following a pilot study, we selected 13 candidate miRNAs and determined their circulating levels in DN (n = 21), SPK-patients (n = 37), healthy controls (n = 19), type 1 diabetes mellitus patients (n = 15) and DN patients with a kidney transplant (n = 15). For validation of selected miRNAs, 14 DN patients were studied longitudinally up to 12 months after SPK. We demonstrated a direct association of miR-25, -27a, -126, -130b, -132, -152, -181a, -223, -320, -326, -340, -574-3p and -660 with DN. Of those, miR-25, -27a, -130b, -132, -152, -320, -326, -340, -574-3p and -660 normalized after SPK. Importantly, circulating levels of some of these miRNAs tightly associate with microvascular impairment as they relate to aberrant capillary tortuosity, angiopoietin-2/angiopoietin-1 ratios, circulating levels of soluble-thrombomodulin and insulin-like growth factor. Taken together, circulating miRNA profiles associate with DN and systemic microvascular damage, and might serve to identify individuals at risk of experiencing microvascular complications, as well as give insight into underlying pathologies.
- Subjects :
- Adult
Male
Oncology
Pathology
medicine.medical_specialty
Adolescent
Disease
Diabetic nephropathy
Young Adult
Internal medicine
microRNA
medicine
Humans
Immunology and Allergy
Diabetic Nephropathies
Pharmacology (medical)
Transplantation
Type 1 diabetes
business.industry
Middle Aged
medicine.disease
Kidney Transplantation
Pathophysiology
MicroRNAs
Circulating MicroRNA
Biomarker (medicine)
Female
Pancreas Transplantation
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- American Journal of Transplantation, 15(4), 1081-1090
- Accession number :
- edsair.doi.dedup.....8198e321b51f9241d2e7c5a132832a55