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The heterotrimeric G protein β subunit RGB1 is required for seedling formation in rice
- Source :
- Rice, Vol 12, Iss 1, Pp 1-14 (2019), Rice
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Background The heterotrimeric G protein β subunit RGB1 plays an important role in plant growth and development. However, the molecular mechanisms underlying the regulation of rice growth by RGB1 remain elusive. Results Here, the rgb1 mutants rgb1–1 (+ 1 bp), rgb1–2 (− 1 bp), and rgb1–3 (− 11 bp) were isolated using the CRISPR/Cas9 system, and they were arrested at 1 day after germination and ultimately exhibited seedling lethality. The dynamic anatomical characteristics of the embryos of the rgb1 seedlings and WT during early postgermination and according to TUNEL assays showed that the suppressed growth of the rgb1 mutants was caused by cell death. In addition to the limited shoot and root development, the development of the embryo shoot-root axis was suppressed in the rgb1 mutants. RGB1 was expressed mainly in the root epidermal and vascular tissues of the embryo. Moreover, transcript profiling analysis revealed that the expression of a large number of auxin-, cytokinin-, and brassinosteroid-inducible genes was upregulated or downregulated in the rgb1 mutant compared to the wild type during seedling development. Conclusions Overall, the rgb1 mutants provide an ideal material for exploring the molecular mechanism underlying rice seedling formation during early postgermination development by G proteins. Significance statement The heterotrimeric G protein β subunit RGB1 acts as a crucial factor in promoting early postgermination seedling development in rice. Electronic supplementary material The online version of this article (10.1186/s12284-019-0313-y) contains supplementary material, which is available to authorized users.
- Subjects :
- Cell death
0106 biological sciences
0301 basic medicine
G protein
Mutant
Soil Science
Plant Science
lcsh:Plant culture
Early postgermination development
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Auxin
RGB1
Heterotrimeric G protein
lcsh:SB1-1110
chemistry.chemical_classification
biology
Wild type
food and beverages
Embryo
biology.organism_classification
Cell biology
030104 developmental biology
chemistry
Seedling
Cytokinin
Original Article
Rice
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 19398433 and 19398425
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Rice
- Accession number :
- edsair.doi.dedup.....81919014200a146e88f21a6a70f46c89