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Disruption of the filamentous actin cytoskeleton is necessary for the activation of capacitative calcium entry in naive smooth muscle cells
- Source :
- Cellular signalling. 17(5)
- Publication Year :
- 2004
-
Abstract
- It has been proposed that cytoskeleton plays a key positive role in the activation of capacitative calcium entry (CCE), which supported the secretion-like hypothesis for the mechanisms underlying this process. However, its role on CCE in native smooth muscle is unknown. Here we demonstrate that CCE in isolated gallbladder myocytes was enhanced by cytochalasin D or latrunculin A treatments (agents that cause actin disassembly) whereas it was reduced by jasplakinolide treatment (which causes actin polymerization), suggesting that actin cytoskeleton acts as a barrier in CCE. In addition, we show for the first time that depletion of intracellular Ca2+ stores by thapsigargin and cholecystokinin in BAPTA-loaded cells induced a decrease in F-actin content that was consistent with a link between CCE and actin reorganization. In conclusion, these data suggest an active participation of actin reorganization in the implementation of CCE and support a conformational coupling model for this process in naive smooth muscle cells.
- Subjects :
- Male
Cytochalasin D
Guinea Pigs
macromolecular substances
Biology
Filamentous actin
Actin remodeling of neurons
Depsipeptides
Myocyte
Animals
Cytoskeleton
Actin
Ion Transport
Actin remodeling
Gallbladder
Muscle, Smooth
Cell Biology
Actin cytoskeleton
Bridged Bicyclo Compounds, Heterocyclic
Actins
Cell biology
Actin Cytoskeleton
Thiazoles
Profilin
biology.protein
Thiazolidines
Calcium
Subjects
Details
- ISSN :
- 08986568
- Volume :
- 17
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Cellular signalling
- Accession number :
- edsair.doi.dedup.....818dabfe4e4b73ffaa7759c52dbfd3d1