Back to Search
Start Over
YidC is in involved in the biogenesis of the secreted autotransporter Hemoglobin Protease
- Source :
- Jong, W S P, ten Hagen-Jongman ten, C M, Ruijter, E, Orru, R V A, Genevaux, P & Luirink, S 2010, ' YidC is in involved in the biogenesis of the secreted autotransporter Hemoglobin Protease ', Journal of Biological Chemistry, vol. 285, no. 51, pp. 39682-39690 . https://doi.org/10.1074/jbc.M110.167650, Journal of Biological Chemistry, 285(51), 39682-39690. American Society for Biochemistry and Molecular Biology Inc.
- Publication Year :
- 2010
-
Abstract
- Autotransporters (ATs) constitute an important family of virulence factors secreted by Gram-negative bacteria. Following their translocation across the inner membrane (IM), ATs temporarily reside in the periplasmic space after which they are secreted into the extracellular environment. Previous studies have shown that the AT hemoglobin protease (Hbp) of Escherichia coli requires a functional signal recognition particle pathway and Sec translocon for optimal targeting to and translocation across the IM. Here, we analyzed the mode of IM translocation of Hbp in more detail. Using site-directed photocross-linking, we found that the Hbp signal peptide is adjacent to YidC early during biogenesis. Notably, YidC is in part associated with the Sec translocon but has until now primarily been implicated in the biogenesis of IM proteins. In vivo, YidC appeared critical for the biogenesis of the ATs Hbp and EspC. For Hbp, depletion of YidC resulted in the formation of secretion-incompetent intermediates that were sensitive to degradation by the periplasmic protease DegP, indicating that YidC activity affects Hbp biogenesis at a late stage, after translocation across the IM. This is the first demonstration of a role for YidC in the biogenesis of an extracellular protein. We propose that YidC is required for maintenance of the translocation-competent state of certain ATs in the periplasm. The large periplasmic domain of YidC is not critical for this novel functionality as it can be deleted without affecting Hbp biogenesis. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
- Subjects :
- Signal peptide
Biology
Protein Sorting Signals
Biochemistry
03 medical and health sciences
Bacterial Proteins
Membrane Biology
Endopeptidases
Escherichia coli
Molecular Biology
Heat-Shock Proteins
030304 developmental biology
Adenosine Triphosphatases
0303 health sciences
Signal recognition particle
SecA Proteins
030306 microbiology
Membrane transport protein
Escherichia coli Proteins
Cell Membrane
Serine Endopeptidases
Membrane Transport Proteins
Cell Biology
Periplasmic space
Translocon
Cell biology
Protein Structure, Tertiary
Periplasm
biology.protein
Periplasmic Proteins
SEC Translocation Channels
Biogenesis
Autotransporters
Subjects
Details
- Language :
- English
- ISSN :
- 00219258
- Database :
- OpenAIRE
- Journal :
- Jong, W S P, ten Hagen-Jongman ten, C M, Ruijter, E, Orru, R V A, Genevaux, P & Luirink, S 2010, ' YidC is in involved in the biogenesis of the secreted autotransporter Hemoglobin Protease ', Journal of Biological Chemistry, vol. 285, no. 51, pp. 39682-39690 . https://doi.org/10.1074/jbc.M110.167650, Journal of Biological Chemistry, 285(51), 39682-39690. American Society for Biochemistry and Molecular Biology Inc.
- Accession number :
- edsair.doi.dedup.....81841d54c93248185e1cc570638318d4