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Fenton Reaction-Induced Oxidative Damage to Membrane Lipids and Protective Effects of 17β-Estradiol in Porcine Ovary and Thyroid Homogenates
- Source :
- International Journal of Environmental Research and Public Health, Vol 17, Iss 6841, p 6841 (2020), International Journal of Environmental Research and Public Health
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The Fenton reaction (Fe2++H2O2→Fe3++•OH+OH-) results in strong oxidative damage to macromolecules when iron (Fe) or hydrogen peroxide (H2O2) are in excess. This study aims at comparing Fe2++H2O2-induced oxidative damage to membrane lipids (lipid peroxidation, LPO) and protective effects of 17β-estradiol (a potential antioxidant) in porcine ovary and thyroid homogenates. Iron, as one of the Fenton reaction substrates, was used in the highest achievable concentrations. Thyroid or ovary homogenates were incubated in the presence of: (1st) FeSO4+H2O2 with/without 17β-estradiol (1 mM; 100, 10.0, 1.0 µM; 100, 10.0, 1.0 nM; 100, 10.0, 1.0 pM); five experiments were performed with different FeSO4 concentrations (2400, 1200, 600, 300, 150 µM); (2nd) FeSO4 (2400, 1200, 600, 300, 150 µM)+H2O2 with/without 17β-estradiol; three experiments were performed with three highest 17β-estradiol concentrations; (3rd) FeSO4 (2400, 1200, 1100, 1000, 900, 800, 700, 600, 300, 150, 75 µM)+H2O2 (5 mM). LPO level [MDA+4-HDA/mg protein] was measured spectrophotometrically. The basal LPO level is lower in ovary than in thyroid homogenates. However, experimentally-induced LPO was higher in the former tissue, which was confirmed for the three highest Fe2+ concentrations (2400, 1200, 1100 µM). Exogenous 17β-estradiol (1 mM, 100, and 10 µM) reduced experimentally-induced LPO independently of iron concentration and that protective effect did not differ between tissues. The ovary, compared to the thyroid, reveals higher sensitivity to prooxidative effects of iron, however, it showed similar responsivity to protective 17β-estradiol activity. The therapeutic effect of 17β-estradiol against iron overload consequences should be considered with relation to both tissues.
- Subjects :
- inorganic chemicals
medicine.medical_specialty
Antioxidant
Swine
Health, Toxicology and Mutagenesis
medicine.medical_treatment
Membrane lipids
Thyroid Gland
lcsh:Medicine
Ovary
oxidative damage
Ferrous
Fenton reaction
Lipid peroxidation
Membrane Lipids
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
medicine
Animals
Hydrogen peroxide
030304 developmental biology
0303 health sciences
Estradiol
Communication
ferrous
lcsh:R
Thyroid
17β-estradiol
Public Health, Environmental and Occupational Health
lipid peroxidation
Hydrogen Peroxide
Oxidative Stress
medicine.anatomical_structure
Endocrinology
chemistry
030220 oncology & carcinogenesis
Female
Subjects
Details
- ISSN :
- 16604601
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- International Journal of Environmental Research and Public Health
- Accession number :
- edsair.doi.dedup.....817967841a183d282bce9f4793dabe1c