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Evaluation of the BD Phoenix M50 Automated Microbiology System for Antimicrobial Susceptibility Testing with Clinical Isolates in Korea
- Source :
- Microbial Drug Resistance. 25:1142-1148
- Publication Year :
- 2019
- Publisher :
- Mary Ann Liebert Inc, 2019.
-
Abstract
- The objectives of this study were to evaluate the performance of the BD Phoenix™ M50 system with two antimicrobial susceptibility testing (AST) panels against clinical isolates in South Korea and the accuracy of determining carbapenem and colistin susceptibility compared with reference methods. A total of 825 nonduplicated clinical isolates were included in this study. Bacterial identification was performed using Bruker Biotyper and 16S rDNA sequencing. Antimicrobial susceptibilities were tested by disk diffusion, broth microdilution, and agar dilution methods. AST with the Phoenix system was performed following the manufacturer's instructions. The categorical agreement (CA), very major error (VME), major error (ME), and minor error (mE) rates were calculated for each antibiotic. CA rates between the results of the Phoenix system and reference methods were more than 90% for most antibiotics except for ciprofloxacin in enterococci (82.7%, 163/197) and cefepime in Acinetobacter species (88.9%, 88/99). VME and ME rates were less than 3% for all the antibiotics tested in this study. Minimum inhibitory concentration (MIC) values for carbapenem and colistin determined by the Phoenix system were highly correlated with those of dilution methods, exhibiting 99.2% (384/387), 96.7% (374/387), and 98.5% (129/131) of the agreement rate within onefold dilution difference for imipenem, meropenem, and colistin, respectively. The BD Phoenix M50™ system showed reliable performance for AST in clinical microbiology laboratories and for detecting carbapenem and colistin resistance in Gram-negative clinical isolates.
- Subjects :
- Microbiology (medical)
Carbapenem
Imipenem
Cefepime
Immunology
Microbial Sensitivity Tests
Biology
Microbiology
Meropenem
Agar dilution
03 medical and health sciences
Minimum inhibitory concentration
RNA, Ribosomal, 16S
Republic of Korea
polycyclic compounds
medicine
Humans
030304 developmental biology
Automation, Laboratory
Pharmacology
0303 health sciences
Acinetobacter
030306 microbiology
Broth microdilution
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Anti-Bacterial Agents
Colistin
Enterococcus
medicine.drug
Subjects
Details
- ISSN :
- 19318448 and 10766294
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Microbial Drug Resistance
- Accession number :
- edsair.doi.dedup.....815def1cb0c1c01b58be73ca7aaa9a8e