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Selective and independent associations of phospholipid transfer protein and hepatic lipase with the LDL subfraction distribution
- Source :
- Journal of Lipid Research, Vol 43, Iss 8, Pp 1256-1263 (2002)
- Publication Year :
- 2002
- Publisher :
- Elsevier, 2002.
-
Abstract
- Phospholipid transfer protein (PLTP), hepatic lipase (HL), and lipoprotein lipase (LPL) have all been reported to be intricately involved in HDL metabolism but the effect of PLTP on the apolipoprotein B-containing lipoproteins relative to that of HL and LPL has not been established. Due to our previous observation of a positive correlation of PLTP activity with plasma apoB and LDL cholesterol, the relationship of PLTP with the LDL subfractions was investigated and compared with that of HL and LPL. Plasma lipoproteins from 50 premenopausal women were fractionated by density gradient ultracentrifugation. Correlations were calculated between the cholesterol concentration of each fraction and plasma PLTP, HL, and LPL activity. Plasma PLTP activity was highly, positively, and selectively correlated with the cholesterol concentration of the buoyant LDL/dense IDL fractions, yet demonstrated a complete absence of an association with the dense LDL fractions. In contrast, HL was positively correlated with the dense LDL fractions but showed no association with buoyant LDL. LPL was also positively correlated with several buoyant LDL fractions; however, the correlations were weaker than those of PLTP. PLTP and LPL were positively correlated and HL was negatively correlated with HDL fractions. The results suggest that PLTP and HL may be important and independent determinants of the LDL subpopulation density distributions.
- Subjects :
- Lipoprotein lipase
Apolipoprotein B
biology
HDL
Cholesterol
lipoprotein distribution
nutritional and metabolic diseases
lipoprotein lipase
Cell Biology
QD415-436
Biochemistry
chemistry.chemical_compound
Endocrinology
High-density lipoprotein
chemistry
high density lipoprotein
Low-density lipoprotein
Phospholipid transfer protein
biology.protein
Density gradient ultracentrifugation
lipids (amino acids, peptides, and proteins)
Hepatic lipase
low density lipoprotein
Subjects
Details
- Language :
- English
- ISSN :
- 00222275
- Volume :
- 43
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research
- Accession number :
- edsair.doi.dedup.....815cc98a4d25ab985251a923732b107e