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Circulating endothelial progenitor cells (EPC) for tumor vasculogenesis in gastric cancer patients
- Source :
- Cancer Letters. 288:124-132
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- It has been suggested that vasculogenesis by endothelial progenitor cells (EPC) as well as angiogenesis play an important role in the production of blood vessels in neoplasm. The present study was designed to isolate and characterize the EPC in gastric cancer patients as a tumor specific angiogenesis marker. The cells derived from CD34 positive PBMC presented with a cobblestone appearance at 28 days, revealing differentiation into endothelial cells. They were also positive to the LDL-uptake reaction, showing that they have biological endothelial cell functions. These cells demonstrated tube formation, showing their ability to participate in neovascularization. The cells derived from CD34 positive PBMC expressed CD133 and demonstrated telomerase activity, showing the stem cell character. In xenograft model, EPC derived from CD34 positive PBMC mobilized mainly into tumor area after being injected through tail vein. With isolation, ex vivo amplification and characterization of EPC from gastric cancer patients receiving chemotherapy, endothelial progenitor cells may be used as a candidate prognostic and predictive biomarker for cancer.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Time Factors
Angiogenesis
Cellular differentiation
CD34
Mice, Nude
Antigens, CD34
Cell Separation
Biology
Neovascularization
Mice
Vasculogenesis
Antigens, CD
Cell Movement
Stomach Neoplasms
Cell Line, Tumor
Antineoplastic Combined Chemotherapy Protocols
medicine
Animals
Humans
AC133 Antigen
Progenitor cell
Telomerase
Cell Proliferation
Glycoproteins
Mice, Inbred BALB C
Neovascularization, Pathologic
Stem Cells
Endothelial Cells
Biological Transport
Cell Differentiation
Lipoproteins, LDL
Endothelial stem cell
Oncology
cardiovascular system
Female
Stem cell
medicine.symptom
Peptides
Biomarkers
Stem Cell Transplantation
Subjects
Details
- ISSN :
- 03043835
- Volume :
- 288
- Database :
- OpenAIRE
- Journal :
- Cancer Letters
- Accession number :
- edsair.doi.dedup.....815768993e10ec989d83629c430c11ba