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Circular RNA circ_0068,888 protects against lipopolysaccharide-induced HK-2 cell injury via sponging microRNA-21–5p
- Source :
- Biochemical and Biophysical Research Communications. 540:1-7
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Our previous findings revealed that hsa_circ_0068,888 was markedly down-regulated in the plasma of patients with sepsis-associated acute kidney injury (AKI). However, its molecular mechanism in AKI remains unclear. Herein, we explored the role of hsa_circ_0068,888 in AKI. Human renal proximal tubular cell line HK-2 was stimulated with lipopolysaccharide (LPS) to mimic AKI in vitro. Decreased hsa_circ_0068,888 expression was observed in AKI cell model. The overexpression of hsa_circ_0068,888 significantly increased the viability of LPS-stimulated HK-2 cells, whereas hsa_circ_0068,888 downregulation showed the opposite effect. Furthermore, LPS triggered inflammatory response and oxidative stress, which was inhibited by hsa_circ_0068,888 overexpression and enhanced by hsa_circ_0068,888 down-regulation. Hsa_circ_0068,888 overexpression suppressed the activation of nuclear factor-κB (NF-κB) pathway triggered by LPS as evidenced by decreased p-p65 protein level and nuclear translocation of p65 in hsa_circ_0068,888 overexpressed cells. Additionally, we proved that hsa_circ_0068,888 targeted microRNA-21–5p (miR-21–5p). The expression of miR-21–5p was markedly increased and was negatively regulated by hsa_circ_0068,888 in LPS-stimulated HK-2 cells. Furthermore, we demonstrated that miR-21–5p overexpression reversed the effects on cell viability, inflammatory response, oxidative stress, and NF-κB pathway induced by hsa_circ_0068,888 overexpression in LPS-stimulated HK-2 cells. Overall, these results implied that hsa_circ_0068,888 shows a protective effect on AKI by sponging miR-21–5p. Hence, up-regulation of hsa_circ_0068,888 might be a potential strategy in treatment for AKI.
- Subjects :
- Lipopolysaccharides
0301 basic medicine
Lipopolysaccharide
Cell Survival
Inflammatory response
Biophysics
medicine.disease_cause
Biochemistry
Cell Line
Kidney Tubules, Proximal
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Downregulation and upregulation
Circular RNA
microRNA
medicine
Humans
Viability assay
Molecular Biology
Inflammation
Base Sequence
NF-kappa B
Acute kidney injury
RNA, Circular
Cell Biology
Acute Kidney Injury
medicine.disease
Molecular biology
MicroRNAs
Oxidative Stress
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Oxidative stress
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 540
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....814b84be124a49e065d72223159c8007