Back to Search
Start Over
Application of NeuronâSelective Fluorescent Probe, NeuA, To Identify Mouse Retinal Degeneration
- Source :
- ChemBioChem. 22:1915-1919
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- The retina is part of the central nerve system (CNS) and has various interneurons and sensory neurons such as photoreceptor cells. Retinitis pigmentosa (RP) is an inherited condition that is characterized by photoreceptor degeneration. Herein, we developed a fluorescent probe - NeuA - for detecting retinal neuronal cells and applied NeuA to discriminate between healthy and RP retinas. The staining pattern of NeuA in the retinas of healthy and RP mouse models was examined in vitro , ex vivo and in vivo using confocal microscopy, the fluorescent fundus microscopy and optical coherent tomography (OCT). NeuA strongly stained the outer segment layer of photoreceptor cells and some bipolar cells in the healthy retina, but there was only weak staining in the photoreceptor degenerated retinas. Therefore, NeuA probe can be used as the detecting RP tools in the preclinical conditions.
- Subjects :
- Retinal degeneration
genetic structures
Mice, Inbred Strains
010402 general chemistry
01 natural sciences
Biochemistry
law.invention
Mice
chemistry.chemical_compound
Confocal microscopy
law
In vivo
Retinitis pigmentosa
medicine
Animals
Molecular Biology
Fluorescent Dyes
Mice, Knockout
Neurons
Retina
Symporters
010405 organic chemistry
Retinal Degeneration
Organic Chemistry
Retinal
medicine.disease
eye diseases
0104 chemical sciences
Cell biology
medicine.anatomical_structure
chemistry
Molecular Medicine
sense organs
Neuron
Ex vivo
Photoreceptor Cells, Vertebrate
Subjects
Details
- ISSN :
- 14397633 and 14394227
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- ChemBioChem
- Accession number :
- edsair.doi.dedup.....812e2a3cf8d43cd39d0b5f2d84a0cfa9