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Interaction of Neisseria gonorrhoeae with classical complement components, C1-inhibitor, and a monoclonal antibody directed against the Neisserial H.8 antigen
- Source :
- Scopus-Elsevier
- Publication Year :
- 1989
- Publisher :
- American Society for Clinical Investigation, 1989.
-
Abstract
- Strains of Neisseria gonorrhoeae were used to evaluate bactericidal and opsonic properties of McAb 10 directed against the Neisserial outer membrane antigen, H.8. Gonococci were either serum resistant in the absence but serum sensitive in the presence, of McAb 10, or serum sensitive or serum resistant regardless of the presence of McAb 10. Strain JS3, which fell in the former category, was used in subsequent studies. C1 zymogen formed by reassociation of isolated C1 subunits was not directly activated by JS3 in the presence or absence of C1-inhibitor. JS3 thus was unable to directly activate the classical pathway independently of antibody. When purified classical pathway components were used to deposit C3 on JS3 in the absence of serum regulatory proteins or antibodies, added C1-inhibitor reduced C3 binding to background levels. When McAb 10 was present, C3 binding was unaffected by C1-inhibitor. Covalently bound, large molecular weight C3 alpha-chain-gonococcal complexes were disbanded by methylamine release of ester linkages. Released 125I-C3 migrated as C3b without degradation by gonococcal proteases. Purified classical components alone or McAb 10 alone facilitated JS3 killing by neutrophils; when combined, the two provided maximal killing. Levels of McAb 10 that only slightly increase C3 deposition on JS3 are bactericidal in serum and maximally opsonic in combination with purified classical pathway components.
- Subjects :
- Blood Bactericidal Activity
Proteases
Neutrophils
medicine.drug_class
Complement C1 Inactivator Proteins
Biology
Monoclonal antibody
Classical complement pathway
Phagocytosis
Antigen
Complement C1
Zymogen
medicine
Humans
Complement Pathway, Classical
Opsonin
Antibodies, Monoclonal
General Medicine
Neisseria gonorrhoeae
Molecular Weight
Biochemistry
biology.protein
Antibody
Research Article
Bacterial Outer Membrane Proteins
Subjects
Details
- ISSN :
- 00219738
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Investigation
- Accession number :
- edsair.doi.dedup.....811dc97243f21b95411268544c0db386
- Full Text :
- https://doi.org/10.1172/jci113897