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Subpallial Enhancer Transgenic Lines: a Data and Tool Resource to Study Transcriptional Regulation of GABAergic Cell Fate
- Source :
- Neuron. 92:59-74
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Elucidating the transcriptional circuitry controlling forebrain development requires an understanding of enhancer activity and regulation. We generated stable transgenic mouse lines that express CreERT2 and GFP from ten different enhancer elements with activity in distinct domains within the embryonic basal ganglia. We used these unique tools to generate a comprehensive regional fate map of the mouse subpallium, including sources for specific subtypes of amygdala neurons. We then focused on deciphering transcriptional mechanisms that control enhancer activity. Using machine-learning computations, in vivo chromosomal occupancy of 13 transcription factors that regulate subpallial patterning and differentiation and analysis of enhancer activity in Dlx1/2 and Lhx6 mutants, we elucidated novel molecular mechanisms that regulate region-specific enhancer activity in the developing brain. Thus, these subpallial enhancer transgenic lines are data and tool resources to study transcriptional regulation of GABAergic cell fate.
- Subjects :
- 0301 basic medicine
Genetically modified mouse
LIM-Homeodomain Proteins
Mice, Transgenic
Nerve Tissue Proteins
Cell fate determination
Biology
Basal Ganglia
Article
Mice
03 medical and health sciences
Fate mapping
Transcriptional regulation
Animals
GABAergic Neurons
Enhancer
Transcription factor
Homeodomain Proteins
Genetics
General Neuroscience
Gene Expression Regulation, Developmental
Cell Differentiation
Embryonic stem cell
Cell biology
Enhancer Elements, Genetic
030104 developmental biology
Forebrain
Transcription Factors
Subjects
Details
- ISSN :
- 08966273
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Neuron
- Accession number :
- edsair.doi.dedup.....810c3abc3e92de8e3c371b35d1593162
- Full Text :
- https://doi.org/10.1016/j.neuron.2016.09.027