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Brassinosteroid Involvement in Arabidopsis thaliana Stomatal Opening
- Source :
- Plant and Cell Physiology. 58:1048-1058
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- Stomata within the plant epidermis regulate CO2 uptake for photosynthesis and water loss through transpiration. Stomatal opening in Arabidopsis thaliana is determined by various factors, including blue light as a signal and multiple phytohormones. Plasma membrane transporters, including H+-ATPase, K+ channels and anion channels in guard cells, mediate these processes, and the activities and expression levels of these components determine stomatal aperture. However, the regulatory mechanisms involved in these processes are not fully understood. In this study, we used infrared thermography to isolate a mutant defective in stomatal opening in response to light. The causative mutation was identified as an allele of the brassinosteroid (BR) biosynthetic mutant dwarf5. Guard cells from this mutant exhibited normal H+-ATPase activity in response to blue light, but showed reduced K+ accumulation and inward-rectifying K+ (K+in) channel activity as a consequence of decreased expression of major K+in channel genes. Consistent with these results, another BR biosynthetic mutant, det2-1, and a BR receptor mutant, bri1-6, exhibited reduced blue light-dependent stomatal opening. Furthermore, application of BR to the hydroponic culture medium completely restored stomatal opening in dwarf5 and det2-1 but not in bri1-6. However, application of BR to the epidermis of dwarf5 did not restore stomatal response. From these results, we conclude that endogenous BR acts in a long-term manner and is required in guard cells with the ability to open stomata in response to light, probably through regulation of K+in channel activity.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Mutant
Arabidopsis
Endogeny
Plant Science
Photosynthesis
01 natural sciences
Plant Epidermis
03 medical and health sciences
chemistry.chemical_compound
Gene Expression Regulation, Plant
Guard cell
Brassinosteroids
Botany
Brassinosteroid
Arabidopsis thaliana
Transpiration
Epidermis (botany)
biology
Arabidopsis Proteins
Chemistry
Cell Membrane
fungi
Cell Biology
General Medicine
biology.organism_classification
Cell biology
Proton-Translocating ATPases
030104 developmental biology
Plant Stomata
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14719053 and 00320781
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Plant and Cell Physiology
- Accession number :
- edsair.doi.dedup.....80fe743ba1e0c14423489d9390c7343c