Back to Search
Start Over
Comparative Analysis of E2F Family Member Oncogenic Activity
- Source :
- PLoS ONE, PLoS ONE, Vol 2, Iss 9, p e912 (2007)
- Publication Year :
- 2007
- Publisher :
- Public Library of Science, 2007.
-
Abstract
- The E2F family of transcription factors consists of nine members with both distinct and overlapping functions. These factors are situated downstream of growth factor signaling cascades, where they play a central role in cell growth and proliferation through their ability to regulate genes involved in cell cycle progression. For this reason, it is likely that the members of the E2F family play a critical role during oncogenesis. Consistent with this idea is the observation that some tumors exhibit deregulated expression of E2F proteins. In order to systematically compare the oncogenic capacity of these family members, we stably over-expressed E2F1 through 6 in non-transformed 3T3 fibroblasts and assessed the ability of these transgenic cell lines to grow under conditions of low serum, as well as to form colonies in soft agar. Our results show that these six E2F family members can be divided into three groups that exhibit differential oncogenic capacity. The first group consists of E2F2 and E2F3a, both of which have strong oncogenic capacity. The second group consists of E2F1 and E2F6, which were neutral in our assays when compared to control cells transduced with vector alone. The third group consists of E2F4 and E2F5, which generally act to repress E2F-responsive genes, and in our assays demonstrated a strong capacity to inhibit transformation. Our results imply that the pattern of expression of these six E2F family members in a cell could exert a strong influence over its susceptibility to oncogenic transformation.
- Subjects :
- Cell division
Blotting, Western
Cell Biology/Cell Growth and Division
lcsh:Medicine
Biology
DNA-binding protein
Polymerase Chain Reaction
Culture Media, Serum-Free
Cell Line
03 medical and health sciences
Mice
0302 clinical medicine
E2F1
Animals
Humans
lcsh:Science
E2F4
Transcription factor
030304 developmental biology
E2F2
DNA Primers
Genetics
0303 health sciences
Multidisciplinary
Base Sequence
Cell growth
lcsh:R
3T3 Cells
Oncogenes
Flow Cytometry
E2F Transcription Factors
Oncology
030220 oncology & carcinogenesis
lcsh:Q
Cell Division
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 2
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....80f89f5ef27259a000a1810a5c98f8b5