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Use of differential display to detect changes in gene expression in the intermediate snail host Biomphalaria glabrata upon infection with Schistosoma mansoni
- Source :
- Parasitology. 120
- Publication Year :
- 2000
-
Abstract
- Changes in gene expression in Biomphalaria glabrata following infection with Schistosoma mansoni have been investigated using a modified differential display approach. RNA was extracted from ovotestis, mantle tissue and anterior nephridium of control and exposed snails at 2 time-points (4 h and 24 h) post-exposure and analysed by RNA fingerprinting. A number of transcripts were identified; some novel and some homologous to mRNAs in GenEMBL that were previously unknown in B. glabrata . Down regulation of one 241 bp mRNA expressed sequence fragment – with an open reading frame showing 48% identity to a cytochrome p450 over 80 residues – has been confirmed using semi-quantitative RT-PCR. Preliminary classification of B. glabrata cyp450 sequence shows it to fall into CLAN 2 of the cytochrome p450 superfamily. Differential display has been successful in identifying changes in gene expression in Biomphalaria glabrata upon infection with Schistosoma mansoni and promises to be a useful technique for the investigation of the interaction between host and parasite.
- Subjects :
- Polymerase Chain Reaction
Gene Expression Regulation, Enzymologic
Host-Parasite Interactions
Cytochrome P-450 Enzyme System
Complementary DNA
parasitic diseases
Gene expression
Biomphalaria glabrata
Animals
RNA, Messenger
Phylogeny
Differential display
biology
Biomphalaria
Intermediate host
RNA
Anatomy
Schistosoma mansoni
biology.organism_classification
Molecular biology
Schistosomiasis mansoni
Open reading frame
Infectious Diseases
Gene Expression Regulation
Animal Science and Zoology
Parasitology
Subjects
Details
- ISSN :
- 00311820
- Volume :
- 120
- Database :
- OpenAIRE
- Journal :
- Parasitology
- Accession number :
- edsair.doi.dedup.....80d48cb4ec8c54a803c0005287546d86